Difference between revisions of "UW-Stout/Hydrogen Peroxide FA22"
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==Introduction== | ==Introduction== | ||
| + | |||
| + | We are using hydrogen peroxide to displaying varying growth of yeast cells. In this experiment, we tested many different concentrations then many different yeast cells with different genes knocked out. | ||
| + | |||
| + | |||
==Materials== | ==Materials== | ||
| + | |||
| + | *5000µM hydrogen peroxide solution | ||
| + | **3% hydrogen peroxide | ||
| + | **Sterile water for dillutions | ||
| + | *Wild-type yeast in 2x synthetic complete media at an OD600 of 0.1-0.2 | ||
| + | *Yeast with different knocked out genes | ||
| + | |||
| + | |||
==Equipment== | ==Equipment== | ||
| + | *Molecular Devices SpectraMax Plus 384 Microplate Reader | ||
| + | *Assay plate | ||
| + | *Micropettes | ||
| + | |||
==Protocols== | ==Protocols== | ||
| − | ===Pilot | + | ===Pilot Experiment=== |
===Knockout Experiment=== | ===Knockout Experiment=== | ||
Revision as of 13:52, 29 November 2022
Contents
Introduction
We are using hydrogen peroxide to displaying varying growth of yeast cells. In this experiment, we tested many different concentrations then many different yeast cells with different genes knocked out.
Materials
- 5000µM hydrogen peroxide solution
- 3% hydrogen peroxide
- Sterile water for dillutions
- Wild-type yeast in 2x synthetic complete media at an OD600 of 0.1-0.2
- Yeast with different knocked out genes
Equipment
- Molecular Devices SpectraMax Plus 384 Microplate Reader
- Assay plate
- Micropettes
