UW-Stout/Ethanol FA21

Materials & Equipment

• Ethanol 100%
• Sterile Water
• Well plates
• Pipets
• Pipet Tips
• Vent Hood
• Centrifuge
• Willed type yeast

Safety

• Eye protection
• Latex gloves
• Lab coat
• Refer to the SDS (safety data sheet) for ethanol

Procedures

1. Dilute the ethanol with the equations below
2. Making the ethanol
   • 50% = 100 ul of H2O to 100 ul of ethanol
   • 33.3% =133.4 ul of H2O to 66.6 ul of ethanol
   • 22.2% =155.5 ul of H2O to 44.4 ul of ethanol
   • 18.5% =163 ul of H20 to 37 ul of ethanol
   • 15.4% = 169.2 ul of H2O to 20.8ul of ethanol
   • 12.8% = 174 ul to 25.6ul of ethanol
   • 10% = 180 ul to 20ul of ethanol
   • 8.8% = 182.4 ul to 17.6ul of ethanol
   • 7.4% = 185.2 ul to 14.8ul of ethanol
   • 4.9% = 190 ul to 9.6ul of ethanol
   • 3.2% =193 ul to 6.4 ul of ethanol
   • 1% = 198 ul to 2 ul of ethanol
3. Using the wild type yeast place into well plates with 50ul of yeast solution and 50ul of diluted ethanol

Wield type of yeast

Yeast Knockout strain

Procedure

1. Dilute the ethanol with the equations below
2. Making the ethanol
   • 18.5% =163 ul of H20 to 37 ul of ethanol
3. Using the five different types of yast
   • BY- Wild type yeast
   • 1-YHL012W
   • 2-YMR221C
   • 3-YPR078C
   • 4-YBR184W
   • 5-YBR284W