Difference between revisions of "UW-Stout/Ethanol FA21"
(Created page with "==Equipment== *Ethanol 100% *Sterile Water *Well plates *Pipets *Pipet Tips *Vent Hood *Centrifuge ==Safety== *Eye protection *Latex gloves *Lab coat *Refer to the SDS (safe...") |
|||
| Line 1: | Line 1: | ||
| − | ==Equipment== | + | ==Materials & Equipment== |
*Ethanol 100% | *Ethanol 100% | ||
*Sterile Water | *Sterile Water | ||
| Line 7: | Line 7: | ||
*Vent Hood | *Vent Hood | ||
*Centrifuge | *Centrifuge | ||
| + | *Willed type yeast | ||
==Safety== | ==Safety== | ||
*Eye protection | *Eye protection | ||
| Line 13: | Line 14: | ||
*Refer to the SDS (safety data sheet) for ethanol | *Refer to the SDS (safety data sheet) for ethanol | ||
==Procedures== | ==Procedures== | ||
| + | #Dilute the ethanol with the equations below | ||
| + | #Making the ethanol | ||
| + | #*50% = 100 ul of H2O to 100 ul of ethanol | ||
| + | #*33.3% =133.4 ul of H2O to 66.6 ul of ethanol | ||
| + | #*22.2% =155.5 ul of H2O to 44.4 ul of ethanol | ||
| + | #*18.5% =163 ul of H20 to 37 ul of ethanol | ||
| + | #*15.4% = 169.2 ul of H2O to 20.8ul of ethanol | ||
| + | #*12.8% = 174 ul to 25.6ul of ethanol | ||
| + | #*10% = 180 ul to 20ul of ethanol | ||
| + | #*8.8% = 182.4 ul to 17.6ul of ethanol | ||
| + | #*7.4% = 185.2 ul to 14.8ul of ethanol | ||
| + | #*4.9% = 190 ul to 9.6ul of ethanol | ||
| + | #*3.2% =193 ul to 6.4 ul of ethanol | ||
| + | #*1% = 198 ul to 2 ul of ethanol | ||
| + | #Using the wield type yeast place into well plates with 50ul of yeast solution and 50ul of diluted ethanol | ||
| + | ==Wield type of yeast== | ||
Revision as of 00:18, 19 December 2021
Materials & Equipment
- Ethanol 100%
- Sterile Water
- Well plates
- Pipets
- Pipet Tips
- Vent Hood
- Centrifuge
- Willed type yeast
Safety
- Eye protection
- Latex gloves
- Lab coat
- Refer to the SDS (safety data sheet) for ethanol
Procedures
- Dilute the ethanol with the equations below
- Making the ethanol
- 50% = 100 ul of H2O to 100 ul of ethanol
- 33.3% =133.4 ul of H2O to 66.6 ul of ethanol
- 22.2% =155.5 ul of H2O to 44.4 ul of ethanol
- 18.5% =163 ul of H20 to 37 ul of ethanol
- 15.4% = 169.2 ul of H2O to 20.8ul of ethanol
- 12.8% = 174 ul to 25.6ul of ethanol
- 10% = 180 ul to 20ul of ethanol
- 8.8% = 182.4 ul to 17.6ul of ethanol
- 7.4% = 185.2 ul to 14.8ul of ethanol
- 4.9% = 190 ul to 9.6ul of ethanol
- 3.2% =193 ul to 6.4 ul of ethanol
- 1% = 198 ul to 2 ul of ethanol
- Using the wield type yeast place into well plates with 50ul of yeast solution and 50ul of diluted ethanol
