Difference between revisions of "UW-Stout/Copper FA21"
(Created page with " == Materials & Equipment == *CuSO4 *Sterile Water *1000ul Pipette *50ul Pipette *Centrifuge Tubes *Wild Type Yeast Cells *Yeast Cells with Your Favorite Gene removed. *Vortex...") |
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#Vortex Wild type Yeast Solutions | #Vortex Wild type Yeast Solutions | ||
#Create Solutions with Copper Sulfate and Sterile water at the following Concentrations. | #Create Solutions with Copper Sulfate and Sterile water at the following Concentrations. | ||
| − | # | + | ##20mM (Solution) Copper Sulfate |
##10mM Copper Sulfate | ##10mM Copper Sulfate | ||
##5.0mM Copper Sulfate | ##5.0mM Copper Sulfate | ||
| Line 32: | Line 32: | ||
#Combine 50ul of the Copper Sulfate solution and 50ul of the wild type yeast cells and put them in eight different wells. | #Combine 50ul of the Copper Sulfate solution and 50ul of the wild type yeast cells and put them in eight different wells. | ||
#Take the wells that are prepared with the yeast and the Copper Sulfate and put it in a plate reader and leave it there for 24 hours. | #Take the wells that are prepared with the yeast and the Copper Sulfate and put it in a plate reader and leave it there for 24 hours. | ||
| + | |||
| + | == Interpreting Wild Type Data == | ||
Revision as of 08:26, 15 December 2021
Contents
Materials & Equipment
- CuSO4
- Sterile Water
- 1000ul Pipette
- 50ul Pipette
- Centrifuge Tubes
- Wild Type Yeast Cells
- Yeast Cells with Your Favorite Gene removed.
- Vortex
- Disposable Pipette Tips
- Plate Reader
- Wells
Personal Protective Equipment
- Latex Gloves
- Eye Pro
- Lab Coat
Wild Type Protocol
- Vortex Wild type Yeast Solutions
- Create Solutions with Copper Sulfate and Sterile water at the following Concentrations.
- 20mM (Solution) Copper Sulfate
- 10mM Copper Sulfate
- 5.0mM Copper Sulfate
- 2.0mM Copper Sulfate
- 1.0mM Copper Sulfate
- 0.5mM Copper Sulfate
- 0.2mM Copper Sulfate
- 0.1mM Copper Sulfate
- Combine 50ul of the Copper Sulfate solution and 50ul of the wild type yeast cells and put them in eight different wells.
- Take the wells that are prepared with the yeast and the Copper Sulfate and put it in a plate reader and leave it there for 24 hours.
