UW-Stout/Cold Shock

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Protocol: Cold Shock'

Materials -8 Knockout Yeast Strains - Wild Type Yeast Strain (BY4735) -Pipetter -Bucket filled with ice -Stop watch -96-well assay plate -9, 200 microliter tubes

Purpose Using cold we will shock each type of yeast to see if it is more or less sensitive to the stress we did on a wild type of yeast labeled BY4735.

Procedure Step 1: Gather all 9 different yeast strains and place in the hood along with 9, 200 microliter tubes. Step 2: Pipette out 100 microliter of each yeast strain placing them each into their own 200 microliter tube. *CHANGING TIP OF PIPETTER IN BETWEEN EACH STRAIN*After each strain is in their own tube, they are now ready to be placed on ice. Step 3: Place each tube on ice for 55 minutes. Step 4: After the 55 minutes, transfer each yeast strain into the 96-well assay plate. Each tube gets its own well, and make sure to change tips in between each strain. The 96-well assay plate is ready to be tested.

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