Difference between revisions of "UW-Stout/Cold Shock"
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===Procedure=== | ===Procedure=== | ||
− | #Gather all 9 different yeast strains and place in the hood along with | + | #Gather all 9 different yeast strains and place in the hood along with all 200 microliter tubes. |
#Pipette out 100 microliter of each yeast strain placing them each into their own 200 microliter tube. *CHANGING TIP OF PIPETTER IN BETWEEN EACH STRAIN*After each strain is in their own tube, they are now ready to be placed on ice. | #Pipette out 100 microliter of each yeast strain placing them each into their own 200 microliter tube. *CHANGING TIP OF PIPETTER IN BETWEEN EACH STRAIN*After each strain is in their own tube, they are now ready to be placed on ice. | ||
#Place each tube on ice for 55 minutes. | #Place each tube on ice for 55 minutes. | ||
#After the 55 minutes, transfer each yeast strain into the 96-well assay plate. Each tube gets its own well, and make sure to change tips in between each strain. The 96-well assay plate is ready to be tested. | #After the 55 minutes, transfer each yeast strain into the 96-well assay plate. Each tube gets its own well, and make sure to change tips in between each strain. The 96-well assay plate is ready to be tested. |
Revision as of 16:34, 8 May 2019
Protocol: Cold Shock
Materials
- 8 Knockout Yeast Strains
- Wild Type Yeast Strain (BY4735)
- Pipetter
- Bucket filled with ice
- Stop watch
- 96-Well Assay Plate
- 200 Microliter Tubes(9)
Purpose
Using cold we will shock each type of yeast to see if it is more or less sensitive to the stress we did on a wild type of yeast labeled BY4735.
Procedure
- Gather all 9 different yeast strains and place in the hood along with all 200 microliter tubes.
- Pipette out 100 microliter of each yeast strain placing them each into their own 200 microliter tube. *CHANGING TIP OF PIPETTER IN BETWEEN EACH STRAIN*After each strain is in their own tube, they are now ready to be placed on ice.
- Place each tube on ice for 55 minutes.
- After the 55 minutes, transfer each yeast strain into the 96-well assay plate. Each tube gets its own well, and make sure to change tips in between each strain. The 96-well assay plate is ready to be tested.