Difference between revisions of "UW-Stout/Cold Shock"
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==Protocol: Cold Shock== | ==Protocol: Cold Shock== | ||
| − | + | ===Materials=== | |
| − | + | *8 Knockout Yeast Strains | |
| − | + | *Wild Type Yeast Strain (BY4735) | |
| − | + | *Pipetter | |
| − | + | *Bucket filled with ice | |
| − | + | *Stop watch | |
| − | + | *96-well assay plate | |
| − | + | *9, 200 microliter tubes | |
| − | + | ===Purpose=== | |
Using cold we will shock each type of yeast to see if it is more or less sensitive to the stress we did on a wild type of yeast labeled BY4735. | Using cold we will shock each type of yeast to see if it is more or less sensitive to the stress we did on a wild type of yeast labeled BY4735. | ||
| − | + | ===Procedure=== | |
| − | + | #Gather all 9 different yeast strains and place in the hood along with 9, 200 microliter tubes. | |
| − | + | #Pipette out 100 microliter of each yeast strain placing them each into their own 200 microliter tube. *CHANGING TIP OF PIPETTER IN BETWEEN EACH STRAIN*After each strain is in their own tube, they are now ready to be placed on ice. | |
| − | + | #Place each tube on ice for 55 minutes. | |
| − | + | #After the 55 minutes, transfer each yeast strain into the 96-well assay plate. Each tube gets its own well, and make sure to change tips in between each strain. The 96-well assay plate is ready to be tested. | |
Revision as of 16:31, 8 May 2019
Protocol: Cold Shock
Materials
- 8 Knockout Yeast Strains
- Wild Type Yeast Strain (BY4735)
- Pipetter
- Bucket filled with ice
- Stop watch
- 96-well assay plate
- 9, 200 microliter tubes
Purpose
Using cold we will shock each type of yeast to see if it is more or less sensitive to the stress we did on a wild type of yeast labeled BY4735.
Procedure
- Gather all 9 different yeast strains and place in the hood along with 9, 200 microliter tubes.
- Pipette out 100 microliter of each yeast strain placing them each into their own 200 microliter tube. *CHANGING TIP OF PIPETTER IN BETWEEN EACH STRAIN*After each strain is in their own tube, they are now ready to be placed on ice.
- Place each tube on ice for 55 minutes.
- After the 55 minutes, transfer each yeast strain into the 96-well assay plate. Each tube gets its own well, and make sure to change tips in between each strain. The 96-well assay plate is ready to be tested.
