Difference between revisions of "UW-Stout/Caffeine FA22"
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1. Make a stock solution at a concentration of 0.1 M | 1. Make a stock solution at a concentration of 0.1 M | ||
| − | + | *Add 0.19419 g of caffeine to 10 mL water | |
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**Vortex briefly | **Vortex briefly | ||
2. Make a stock solution at a concentration of 0.05 M | 2. Make a stock solution at a concentration of 0.05 M | ||
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**Take 250 ul of 3.90625 x 10^-4 ul and add 250 ul of water | **Take 250 ul of 3.90625 x 10^-4 ul and add 250 ul of water | ||
| − | **Vortex briefly | + | **Vortex briefly |
===Yeast + Wells=== | ===Yeast + Wells=== | ||
Revision as of 14:12, 22 November 2022
Contents
Yeast Caffeine Torture
Caffeine Solubility: 2g/ 100 mL
Pilot Experiment Protocol
Materials:
- Sterile Water
- Caffeine
- Wild Yeast
- Weigh Boat
- Microcentrifuge tubes
- Ethanol spray to sterilize equipment
Procedure:
Stock Solutions
Underneath the hood
1. Make a stock solution at a concentration of 0.1 M
- Add 0.19419 g of caffeine to 10 mL water
- Vortex briefly
2. Make a stock solution at a concentration of 0.05 M
- Take 250 ul of 0.1 M and add 250 ul water
- Vortex briefly
3. Make a stock solution at a concentration of 0.025 M
- Take 250 ul of 0.05 M and add 250ul of water
- Vortex briefly
4. Make a stock solution at a concentration of 0.0125 M
- Take 250 ul of 0.025 M and add 250 ul water
- Vortex briefly
5. Make a stock solution at a concentration of 0.00625 M
- Take 250 ul of 0.0125 and add 250 ul of water
- Vortex briefly
6. Make a stock solution at a concentration of 0.003125 M
- Take 250 ul of 0.00625 M and add 250 ul of water
- Vortex briefly
7. Make a stock solution at a concentration of 0.0015625 M
- Take 250 ul of 0.003125 M and add 250 ul of water
- Vortex briefly
8. Make a stock solution at a concentration of 7.8125 x 10^-4 M
- Take 250 ul of 0.0015625 M and add 250 ul of water
- Vortex briefly
9. Make a stock solution at a concentration of 3.90625 x 10^-4 M
- Take 250 ul of 7.8125 x 10^-4 M and add 250 ul of water
10. Make a stock solution at a concentration of 1.953125 x 10^-4 M
- Take 250 ul of 3.90625 x 10^-4 ul and add 250 ul of water
- Vortex briefly
Yeast + Wells
- Vortex the yeast briefly before use.
- To the first well, add 50 ul of 0.1 M stock solution and 50 ul of yeast.
- Repeat the first step in the next well for 0.05 M solution, continuing to add each stock solution to the next wells.
Inducing Stress Protocol
Materials
- 10 mL 0.1 M Stock Solution
- Test tubes
- Yeast sample
