Difference between revisions of "UW-Stout/Caffeine FA22"
(→Procedure:) |
(→Procedure:) |
||
| Line 10: | Line 10: | ||
*Ethanol spray to sterilize equipment | *Ethanol spray to sterilize equipment | ||
| − | + | ==Procedure:== | |
| − | + | ===Stock Solutions=== | |
Underneath the hood | Underneath the hood | ||
| − | + | 1. Make a stock solution at a concentration of 0.1 M | |
| + | |||
**Add 0.19419 g of caffeine to 10 mL water | **Add 0.19419 g of caffeine to 10 mL water | ||
**Vortex briefly | **Vortex briefly | ||
| − | + | 2. Make a stock solution at a concentration of 0.05 M | |
| + | |||
**Take 250 ul of 0.1 M and add 250 ul water | **Take 250 ul of 0.1 M and add 250 ul water | ||
**Vortex briefly | **Vortex briefly | ||
3. Make a stock solution at a concentration of 0.025 M | 3. Make a stock solution at a concentration of 0.025 M | ||
| + | |||
**Take 250 ul of 0.05 M and add 250ul of water | **Take 250 ul of 0.05 M and add 250ul of water | ||
**Vortex briefly | **Vortex briefly | ||
4. Make a stock solution at a concentration of 0.0125 M | 4. Make a stock solution at a concentration of 0.0125 M | ||
| + | |||
**Take 250 ul of 0.025 M and add 250 ul water | **Take 250 ul of 0.025 M and add 250 ul water | ||
**Vortex briefly | **Vortex briefly | ||
5. Make a stock solution at a concentration of 0.00625 M | 5. Make a stock solution at a concentration of 0.00625 M | ||
| + | |||
**Take 250 ul of 0.0125 and add 250 ul of water | **Take 250 ul of 0.0125 and add 250 ul of water | ||
**Vortex briefly | **Vortex briefly | ||
6. Make a stock solution at a concentration of 0.003125 M | 6. Make a stock solution at a concentration of 0.003125 M | ||
| + | |||
**Take 250 ul of 0.00625 M and add 250 ul of water | **Take 250 ul of 0.00625 M and add 250 ul of water | ||
**Vortex briefly | **Vortex briefly | ||
7. Make a stock solution at a concentration of 0.0015625 M | 7. Make a stock solution at a concentration of 0.0015625 M | ||
| + | |||
**Take 250 ul of 0.003125 M and add 250 ul of water | **Take 250 ul of 0.003125 M and add 250 ul of water | ||
**Vortex briefly | **Vortex briefly | ||
| − | 8. Make a stock solution at a concentration of 7.8125 x 10 | + | 8. Make a stock solution at a concentration of 7.8125 x 10^-4 M |
| + | |||
| + | **Take 250 ul of 0.0015625 M and add 250 ul of water | ||
| + | **Vortex briefly | ||
| + | 9. Make a stock solution at a concentration of 3.90625 x 10^-4 M | ||
| + | |||
| + | **Take 250 ul of 7.8125 x 10^-4 M and add 250 ul of water | ||
| + | 10. Make a stock solution at a concentration of 1.953125 x 10^-4 M | ||
| + | |||
| + | **Take 250 ul of 3.90625 x 10^-4 ul and add 250 ul of water | ||
| + | **Vortex briefly | ||
| + | |||
| + | ===Yeast + Wells=== | ||
| + | # Vortex the yeast briefly before use. | ||
| + | # To the first well, add 50 ul of 0.1 M stock solution and 50 ul of yeast. | ||
| + | # Repeat the first step in the next well for 0.05 M solution, continuing to add each stock solution to the next wells. | ||
Revision as of 14:04, 22 November 2022
Contents
Yeast Caffeine Torture
Caffeine Solubility: 2g/ 100 mL
Pilot Experiment Protocol
Materials:
- Sterile Water
- Caffeine
- Wild Yeast
- Weigh Boat
- Microcentrifuge tubes
- Ethanol spray to sterilize equipment
Procedure:
Stock Solutions
Underneath the hood
1. Make a stock solution at a concentration of 0.1 M
- Add 0.19419 g of caffeine to 10 mL water
- Vortex briefly
2. Make a stock solution at a concentration of 0.05 M
- Take 250 ul of 0.1 M and add 250 ul water
- Vortex briefly
3. Make a stock solution at a concentration of 0.025 M
- Take 250 ul of 0.05 M and add 250ul of water
- Vortex briefly
4. Make a stock solution at a concentration of 0.0125 M
- Take 250 ul of 0.025 M and add 250 ul water
- Vortex briefly
5. Make a stock solution at a concentration of 0.00625 M
- Take 250 ul of 0.0125 and add 250 ul of water
- Vortex briefly
6. Make a stock solution at a concentration of 0.003125 M
- Take 250 ul of 0.00625 M and add 250 ul of water
- Vortex briefly
7. Make a stock solution at a concentration of 0.0015625 M
- Take 250 ul of 0.003125 M and add 250 ul of water
- Vortex briefly
8. Make a stock solution at a concentration of 7.8125 x 10^-4 M
- Take 250 ul of 0.0015625 M and add 250 ul of water
- Vortex briefly
9. Make a stock solution at a concentration of 3.90625 x 10^-4 M
- Take 250 ul of 7.8125 x 10^-4 M and add 250 ul of water
10. Make a stock solution at a concentration of 1.953125 x 10^-4 M
- Take 250 ul of 3.90625 x 10^-4 ul and add 250 ul of water
- Vortex briefly
Yeast + Wells
- Vortex the yeast briefly before use.
- To the first well, add 50 ul of 0.1 M stock solution and 50 ul of yeast.
- Repeat the first step in the next well for 0.05 M solution, continuing to add each stock solution to the next wells.
