UW-Stout/Caffeine

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Sensitivity to Caffeine

Purpose

The purpose of this lab is to test the sensitivity to the caffeine of knock out strains of yeast cells. We will be analyzing the data on the gene's response to different levels of caffeine concentration.


Materials

  • 10 mM powdered caffeine
  • 100 mM of caffeine solution
  • Wild Type Yeast
  • Knock out genes

Equipment

-Assay Plate -Micropipettes -1.5 mL test tubes -Incubator set at 30 degrees celsius

General Protocol

-Find concentrations of caffeine solution needed for experiment -Combine caffeine solution with Wild Type yeast (BY4735) in assay plate -Incubate at 30 degrees celsius overnight