Difference between revisions of "UW-Stout/Caffeine"
| Line 13: | Line 13: | ||
== Equipment == | == Equipment == | ||
| − | + | * Assay Plate | |
| − | + | * Micropipettes | |
| − | + | * 1.5 mL test tubes | |
| − | + | * Incubator set at 30 degrees celsius | |
== General Protocol == | == General Protocol == | ||
| − | + | * Find concentrations of caffeine solution needed for experiment | |
| − | + | * Combine caffeine solution with Wild Type yeast (BY4735) in assay plate | |
| − | + | * Incubate at 30 degrees celsius overnight | |
Revision as of 07:48, 30 April 2019
Sensitivity to Caffeine
Purpose
The purpose of this lab is to test the sensitivity to the caffeine of knock out strains of yeast cells. We will be analyzing the data on the gene's response to different levels of caffeine concentration.
Materials
- 10 mM powdered caffeine
- 100 mM of caffeine solution
- Wild Type Yeast
- Knock out genes
Equipment
- Assay Plate
- Micropipettes
- 1.5 mL test tubes
- Incubator set at 30 degrees celsius
General Protocol
- Find concentrations of caffeine solution needed for experiment
- Combine caffeine solution with Wild Type yeast (BY4735) in assay plate
- Incubate at 30 degrees celsius overnight
