UW-Stout/Acids SP23
Introduction
We are looking to find a pH that puts yeast under stress. Our end goal is to be able to test different knockout yeast strains against an acidic medium, but to do this we first need to find a pH that allows the yeast to grow, but puts some strain on the growth curve. In this protocol, we are testing wild yeast strains against different concentration of acetic acid. By analyzing the growth curves, we will be able to find a concentration that will stress the yeast growth.
Materials
- BY (wild type) Yeast
- 17.4 M Glacial Acetic Acid
- Deionized Water
- Sterile Water
- Pipettes
- Well Sheet
- 1.7 mL microcentrifuge tubes
- Disposable Gloves
Procedure
- Using 17.4 M Glacial Acetic Acid, create a diluted stock solution
- Use gloves when working with concentrated acid
- Add 1.04 mL of Glacial Acetic Acid to 8.95 mL of Deionized Water
