Difference between revisions of "Strains"
(→Strain Collections) |
|||
| Line 21: | Line 21: | ||
=Strain Collections= | =Strain Collections= | ||
| − | *[ | + | *[https://www.thermofisher.com/us/en/home/references/protocols/proteins-expression-isolation-and-analysis/gfp-protocol/yeast-gfp-clone-collection.html Yeast-GFP Clone Collection] from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, consisting of C-terminal tagged open reading frames (ORFs). These may be available from the [https://mayaschuldiner.wixsite.com/schuldinerlab/swat Schuldiner Lab SWAT]. |
| − | *[http:// | + | *[http://sites.utoronto.ca/andrewslab/data.shtml Yeast GST-Tagged Collection] for inducible overexpression of yeast ORFs, developed in the Andrews lab at the University of Toronto. These may be available from [https://horizondiscovery.com/en/non-mammalian-research-tools/products/yeast-gst-tagged-orfs Horizon]. |
*[http://yeastdeletion-sgtc.yeastgenome.org/downloads.html#strainsavail Yeast Knockout (YKO) Collection] from the [http://yeastdeletion-sgtc.yeastgenome.org/consortium.html <i>Saccharomyces</i> Genome Deletion Consortium]. | *[http://yeastdeletion-sgtc.yeastgenome.org/downloads.html#strainsavail Yeast Knockout (YKO) Collection] from the [http://yeastdeletion-sgtc.yeastgenome.org/consortium.html <i>Saccharomyces</i> Genome Deletion Consortium]. | ||
| − | *[ | + | *[https://horizondiscovery.com/en/non-mammalian-research-tools/products/yeast-tap-tagged-orfs Yeast-TAP Fusion Library] from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, containing open reading frames (ORFs) tagged with a high-affinity epitope and expressed from their natural chromosomal locations. |
*[http://www.openbiosystems.com/GeneExpression/Yeast/Tet-Promoters/ Yeast Tet-promoters Hughes Collection] containing 800 essential yeast genes for which expression is regulated by doxycycline. Hosted by [http://www.openbiosystems.com Open Biosystems]. | *[http://www.openbiosystems.com/GeneExpression/Yeast/Tet-Promoters/ Yeast Tet-promoters Hughes Collection] containing 800 essential yeast genes for which expression is regulated by doxycycline. Hosted by [http://www.openbiosystems.com Open Biosystems]. | ||
*The Yeast Transposon Insertion Library Collection, derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, is available from the [http://snyderlab.stanford.edu/ Mike Snyder lab] at Stanford University. Please contact Minyi Shi (minyishi at stanford dot edu) with requests. | *The Yeast Transposon Insertion Library Collection, derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, is available from the [http://snyderlab.stanford.edu/ Mike Snyder lab] at Stanford University. Please contact Minyi Shi (minyishi at stanford dot edu) with requests. | ||
Revision as of 12:36, 30 June 2023
Strain Backgrounds
- View genotypes and descriptions of commonly used strains.
- Table of commonly used auxotrophic markers, with sequence information where known.
Stock Centers
- American Type Culture Collection (ATCC) maintains yeast stocks and clones.
- EUROSCARF, the EUROpean Saccharomyces Cerevisiae ARchive for Functional analysis, maintains a collection of systematic deletion strains searchable by gene name.
- National Collection of Yeast Cultures (NCYC) maintains over 3,100 non-pathogenic yeasts, including type strains, strains of general interest for education and research, strains of industrial importance, and genetically marked strains.
- Common Access to Biological Resources and Information (CABRI) includes catalogs from European culture collections for yeast and other organisms.
- Addgene is a non-profit plasmid repository that distributes many plasmids for yeast research. In particular, Addgene is distributing a collection of Yeast Advanced Gateway Destination Vectors created by Dr. Susan Lindquist's laboratory.
- Yeast Genetic Resource Center (YGRC) maintains over 4,800 S. pombe strains and over 9,000 S. cerevisiae strains.
- Industrial Yeasts Collection DBVPG. The Industrial Yeasts Collection DBVPG, an academic biological resource centre (BRC) specialized in yeasts and yeast-like microorganisms, distributes strains and offers services to the international scientific community and to other private Institutions. DBVPG maintains over 6,000 yeast strains and over 300 yeast-like (Prototheca sp. ) strains.
Strain Collections
- Yeast-GFP Clone Collection from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, consisting of C-terminal tagged open reading frames (ORFs). These may be available from the Schuldiner Lab SWAT.
- Yeast GST-Tagged Collection for inducible overexpression of yeast ORFs, developed in the Andrews lab at the University of Toronto. These may be available from Horizon.
- Yeast-TAP Fusion Library from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, containing open reading frames (ORFs) tagged with a high-affinity epitope and expressed from their natural chromosomal locations.
- Yeast Tet-promoters Hughes Collection containing 800 essential yeast genes for which expression is regulated by doxycycline. Hosted by Open Biosystems.
- The Yeast Transposon Insertion Library Collection, derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, is available from the Mike Snyder lab at Stanford University. Please contact Minyi Shi (minyishi at stanford dot edu) with requests.
