Difference between revisions of "Strains"

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=Strain Backgrounds=
 
=Strain Backgrounds=
 
*View genotypes and descriptions of [[Commonly_used_strains|commonly used strains]].
 
*View genotypes and descriptions of [[Commonly_used_strains|commonly used strains]].
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*View [http://gbrowse.princeton.edu/cgi-bin/gbrowse/yeast_strains_snps/ SNPs] in <i>S. cerevisiae</i> strains
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*Table of [[Commonly_used_auxotrophic_markers|commonly used auxotrophic markers]], with sequence information where known.
  
 
=Stock Centers=
 
=Stock Centers=
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*[http://www.addgene.org Addgene] is a non-profit plasmid repository that distributes many plasmids for yeast research.  In particular, Addgene is distributing a collection of [http://www.addgene.org/yeast_gateway Yeast Advanced Gateway Destination Vectors] created by Dr. Susan Lindquist's laboratory.
 
*[http://www.addgene.org Addgene] is a non-profit plasmid repository that distributes many plasmids for yeast research.  In particular, Addgene is distributing a collection of [http://www.addgene.org/yeast_gateway Yeast Advanced Gateway Destination Vectors] created by Dr. Susan Lindquist's laboratory.
  
*[http://yeast.lab.nig.ac.jp/nig/index_en.html Yeast Genetic Resource Center (YGRC)] maintains over 4,800 S. pombe strains and over 9,000 S. cerevisiae strains.
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*[http://yeast.lab.nig.ac.jp/yeast/ Yeast Genetic Resource Center (YGRC)] maintains over 4,800 S. pombe strains and over 9,000 S. cerevisiae strains.
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*[http://www.dbvpg.unipg.it/ Industrial Yeasts Collection DBVPG]. The Industrial Yeasts Collection DBVPG, an academic biological resource centre (BRC) specialized in yeasts and yeast-like microorganisms, distributes strains and offers services to the international scientific community and to other private Institutions. DBVPG maintains over 6,000 yeast strains and over 300  yeast-like (Prototheca sp. ) strains.
  
 
=Strain Collections=
 
=Strain Collections=
  
*[http://clones.invitrogen.com/cloneinfo.php?clone=yeastgfp Yeast-GFP Clone Collection] from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, consisting of C-terminal tagged open reading frames (ORFs).  Hosted by [http://www.invitrogen.com Invitrogen]
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*[http://clones.invitrogen.com/cloneinfo.php?clone=yeastgfp Yeast-GFP Clone Collection] from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, consisting of C-terminal tagged open reading frames (ORFs).  Hosted by [http://www.invitrogen.com/site/us/en/home/brands/Invitrogen.html Invitrogen].
  
*[http://www.dbsr.duke.edu/yeast/Genome Libraries/exclones.htm Yeast GST-fusion Collection] developed in [http://www.sciencemag.org/cgi/content/abstract/286/5442/1153 Eric Phizicky's] laboratory. A total of 6080 individual yeast strains, each containing a plasmid expressing a different full-length yeast Open Reading Frame (ORF) fused to a glutathione S-transferase (GST). Hosted by [https://www.resgen.com/products/YEXclns.php3 ResGen Invitrogen]
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*[http://www.thermoscientificbio.com/non-mammalian-cdna-and-orf/yeast-gst-tagged-orfs/ Yeast GST-Tagged Collection] for inducible overexpression of yeast ORFs, developed in the Andrews lab at the University of Toronto. Hosted by [http://www.thermoscientificbio.com/openbiosystems/?redirect=true Open Biosystems].
  
 
*[http://www-sequence.stanford.edu/group/yeast_deletion_project/deletions3.html Yeast Knockout (YKO) Collection] from the [http://www-sequence.stanford.edu/group/yeast_deletion_project/member.html <i>Saccharomyces</i> Genome Deletion Consortium].  
 
*[http://www-sequence.stanford.edu/group/yeast_deletion_project/deletions3.html Yeast Knockout (YKO) Collection] from the [http://www-sequence.stanford.edu/group/yeast_deletion_project/member.html <i>Saccharomyces</i> Genome Deletion Consortium].  
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**[http://www-sequence.stanford.edu/group/yeast_deletion_project/gb/ykocp.html Read] all comments.
 
**[http://www-sequence.stanford.edu/group/yeast_deletion_project/gb/ykocp.html Read] all comments.
  
*[http://www.openbiosystems.com/GeneExpression/Yeast/TAP/ Yeast-TAP Fusion Library] from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, containing open reading frames (ORFs) tagged with a high-affinity epitope and expressed from their natural chromosomal locations. Hosted by [http://www.openbiosystems.com Open Biosystems]
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*[http://www.thermoscientificbio.com/non-mammalian-cdna-and-orf/yeast-tap-tagged-orfs/ Yeast-TAP Fusion Library] from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, containing open reading frames (ORFs) tagged with a high-affinity epitope and expressed from their natural chromosomal locations. Hosted by [http://www.thermoscientificbio.com/openbiosystems/?redirect=true Open Biosystems].
  
 
*[http://www.openbiosystems.com/GeneExpression/Yeast/Tet-Promoters/ Yeast Tet-promoters Hughes Collection] containing 800 essential yeast genes for which expression is regulated by doxycycline. Hosted by [http://www.openbiosystems.com Open Biosystems].
 
*[http://www.openbiosystems.com/GeneExpression/Yeast/Tet-Promoters/ Yeast Tet-promoters Hughes Collection] containing 800 essential yeast genes for which expression is regulated by doxycycline. Hosted by [http://www.openbiosystems.com Open Biosystems].
  
*[http://ygac.med.yale.edu/mtn/insertion_libraries.stm Yeast Transposon Insertion Library Collection], derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, from the [http://ygac.med.yale.edu/default.stm Yale Genome Analysis Center]
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*The Yeast Transposon Insertion Library Collection, derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, is available from the [http://snyderlab.stanford.edu/ Mike Snyder lab] at Stanford University. Please contact Minyi Shi (minyishi at stanford dot edu) with requests.

Latest revision as of 10:27, 5 May 2017

Strain Backgrounds

Stock Centers

  • EUROSCARF, the EUROpean Saccharomyces Cerevisiae ARchive for Functional analysis, maintains a collection of systematic deletion strains searchable by gene name.
  • National Collection of Yeast Cultures (NCYC) maintains over 3,100 non-pathogenic yeasts, including type strains, strains of general interest for education and research, strains of industrial importance, and genetically marked strains.
  • Industrial Yeasts Collection DBVPG. The Industrial Yeasts Collection DBVPG, an academic biological resource centre (BRC) specialized in yeasts and yeast-like microorganisms, distributes strains and offers services to the international scientific community and to other private Institutions. DBVPG maintains over 6,000 yeast strains and over 300 yeast-like (Prototheca sp. ) strains.

Strain Collections

  • Yeast-TAP Fusion Library from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, containing open reading frames (ORFs) tagged with a high-affinity epitope and expressed from their natural chromosomal locations. Hosted by Open Biosystems.
  • The Yeast Transposon Insertion Library Collection, derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, is available from the Mike Snyder lab at Stanford University. Please contact Minyi Shi (minyishi at stanford dot edu) with requests.