Positions in yeast labs
University of Washington Genome Sciences Dept, Seattle, USA
Maitreya Dunham is moving her lab from Princeton to UW this summer, and is looking for postdoc applicants. The Dunham Lab studies evolution and systems biology in yeast from a genomic perspective. Lab interests include
- experimental evolution over a variety of conditions and genotypes
- integrating mutation and gene expression data
- aneuploidy and genome rearrangement effects on fitness and relation with transposons
- comparative genomics using S. bayanus
- gene expression and genome evolution in hybrid yeasts
- technology development for chemostats, whole genome characterization, and other applications
Carnegie Institution for Science, Stanford, California, USA
Highly qualified and motivated individuals are invited to send applications for a Postdoc Position in the Research Group of Wolf B. Frommer on the topic
Regulatory circuits controlling sugar flux in yeast grown on ethanol
HT screen of the yeast knock out collection using FRET sensors for glucose, sucrose and maltose, follow-up analysis of hits and reconstruction of networks
Our lab has developed a wide range of FRET sensors for metabolites. These sensors have so far been used mainly in mammalian cells to study glutamate release from neurons, glucose transport across the ER membrane or tryptophan/kynurenine exchange.
We have now been able to functionally express the FRET sensors also in the cytoplasm of yeast and to establish a high throughput screening platform. Our goal is to identify novel regulatory pathways involved in the control of glucose flux in yeast. As a first step, the kinase k.o. collection has been screened for altered glucose flux and several hits have been identified and verified.
Next steps will be to verify the hits using a new microfluidic platform, to test the effect on other sugar fluxes using FRET sensors for sucrose, maltose and ribose and to place the kinases into signaling networks. The screen can be expanded to include the whole genome at a later stage. Focus points are regulatory effects on glucose transporters and hexokinases. Due to the advanced stage, it is expected that the work will lead to high profile publications within less than a year.
Start date asap
Send your application with CV and the names of three references to:
Wolf B. Frommer Carnegie Institution for Science 260 Panama St, Stanford CA 94305 USA. Website E-mail: email@example.com