https://wiki.yeastgenome.org/index.php?title=GRSandPRIMED&feed=atom&action=historyGRSandPRIMED - Revision history2024-03-29T10:53:25ZRevision history for this page on the wikiMediaWiki 1.31.14https://wiki.yeastgenome.org/index.php?title=GRSandPRIMED&diff=399088&oldid=prevJdemeter at 18:58, 9 February 20152015-02-09T18:58:26Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 18:58, 9 February 2015</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete <u>PRIME</u>r <u>D</u>atabase for deleting and C-terminally tagging every protein-coding gene in the main ''S. pombe'' and five of the most commonly used ''S. cerevisiae'' strains. In addition to protein-coding genes, the authors also provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED has been tested for accuracy and is available for download in Excel format.</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete <u>PRIME</u>r <u>D</u>atabase for deleting and C-terminally tagging every protein-coding gene in the main ''S. pombe'' and five of the most commonly used ''S. cerevisiae'' strains <ins class="diffchange diffchange-inline">(S288C, RM11–1A, SK1, W303, and Y55)</ins>. In addition to protein-coding genes, the authors also provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED has been tested for accuracy and is available for download in Excel format.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated is [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 available] in PLOS ONE.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated is [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 available] in PLOS ONE.</div></td></tr>
</table>Jdemeterhttps://wiki.yeastgenome.org/index.php?title=GRSandPRIMED&diff=399084&oldid=prevJdemeter at 21:10, 5 February 20152015-02-05T21:10:15Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 21:10, 5 February 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l3" >Line 3:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete <u>PRIME</u>r <u>D</u>atabase for deleting and C-<del class="diffchange diffchange-inline">terminal </del>tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, the authors also provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED <del class="diffchange diffchange-inline">have </del>been tested for accuracy and <del class="diffchange diffchange-inline">are provided as downloadable </del>Excel <del class="diffchange diffchange-inline">files</del>.</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete <u>PRIME</u>r <u>D</u>atabase for deleting and C-<ins class="diffchange diffchange-inline">terminally </ins>tagging every protein-coding gene in the main <ins class="diffchange diffchange-inline">''</ins>S. pombe<ins class="diffchange diffchange-inline">'' </ins>and five of the most commonly used <ins class="diffchange diffchange-inline">''</ins>S. cerevisiae<ins class="diffchange diffchange-inline">'' </ins>strains. In addition to protein-coding genes, the authors also provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED <ins class="diffchange diffchange-inline">has </ins>been tested for accuracy and <ins class="diffchange diffchange-inline">is available for download in </ins>Excel <ins class="diffchange diffchange-inline">format</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated is [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 available] in PLOS ONE.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated is [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 available] in PLOS ONE.</div></td></tr>
</table>Jdemeterhttps://wiki.yeastgenome.org/index.php?title=GRSandPRIMED&diff=399083&oldid=prevJdemeter at 20:33, 5 February 20152015-02-05T20:33:58Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 20:33, 5 February 2015</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated <del class="diffchange diffchange-inline">are described in a PLOS ONE </del>[http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 <del class="diffchange diffchange-inline">paper</del>].</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated <ins class="diffchange diffchange-inline">is </ins>[http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 <ins class="diffchange diffchange-inline">available</ins>] <ins class="diffchange diffchange-inline">in PLOS ONE</ins>.</div></td></tr>
</table>Jdemeterhttps://wiki.yeastgenome.org/index.php?title=GRSandPRIMED&diff=399082&oldid=prevJdemeter at 20:32, 5 February 20152015-02-05T20:32:53Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 20:32, 5 February 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l3" >Line 3:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete <u>PRIME</u>r <u>D</u>atabase for deleting and C-terminal tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, <del class="diffchange diffchange-inline">we </del>provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED have been tested for accuracy and are provided as downloadable Excel files.</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete <u>PRIME</u>r <u>D</u>atabase for deleting and C-terminal tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, <ins class="diffchange diffchange-inline">the authors also </ins>provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED have been tested for accuracy and are provided as downloadable Excel files.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated are described in a PLOS ONE [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 paper].</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated are described in a PLOS ONE [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 paper].</div></td></tr>
</table>Jdemeterhttps://wiki.yeastgenome.org/index.php?title=GRSandPRIMED&diff=399081&oldid=prevJdemeter at 20:32, 5 February 20152015-02-05T20:32:02Z<p></p>
<table class="diff diff-contentalign-left" data-mw="interface">
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<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">← Older revision</td>
<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 20:32, 5 February 2015</td>
</tr><tr><td colspan="2" class="diff-lineno" id="mw-diff-left-l3" >Line 3:</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td></tr>
<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete <del class="diffchange diffchange-inline">PRIMEr Database </del>for deleting and C-terminal tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, we provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED have been tested for accuracy and are provided as downloadable Excel files.</div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete <ins class="diffchange diffchange-inline"><u>PRIME</u>r <u>D</u>atabase </ins>for deleting and C-terminal tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, we provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED have been tested for accuracy and are provided as downloadable Excel files.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div><br /></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated are described in a PLOS ONE [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 paper].</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated are described in a PLOS ONE [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 paper].</div></td></tr>
</table>Jdemeterhttps://wiki.yeastgenome.org/index.php?title=GRSandPRIMED&diff=399080&oldid=prevJdemeter at 20:27, 5 February 20152015-02-05T20:27:42Z<p></p>
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<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 20:27, 5 February 2015</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"><br /></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete PRIMEr Database for deleting and C-terminal tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, we provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED have been tested for accuracy and are provided as downloadable Excel files.</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete PRIMEr Database for deleting and C-terminal tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, we provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED have been tested for accuracy and are provided as downloadable Excel files.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;"><br /></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated are described in a PLOS ONE [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 paper].</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>The publication describing the software and the primers it generated are described in a PLOS ONE [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 paper].</div></td></tr>
</table>Jdemeterhttps://wiki.yeastgenome.org/index.php?title=GRSandPRIMED&diff=399079&oldid=prevJdemeter: Created page with "'''Genome Retrieval Script (GRS) and PRIMED''' [http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizabl..."2015-02-05T20:26:46Z<p>Created page with "'''Genome Retrieval Script (GRS) and PRIMED''' [http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizabl..."</p>
<p><b>New page</b></p><div>'''Genome Retrieval Script (GRS) and PRIMED'''<br />
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[http://www.massgeneral.org/cancer/research/motamediresources.aspx Genome Retrieval Script] (GRS) is a Python-based customizable, open source code for genome analysis. GRS comes with detailed directions about how it can be modified to retrieve custom sequences from any annotated genome.<br />
Using GRS, the authors created [http://www.massgeneral.org/cancer/research/motamediresources.aspx PRIMED]: the complete PRIMEr Database for deleting and C-terminal tagging every protein-coding gene in the main S. pombe and five of the most commonly used S. cerevisiae strains. In addition to protein-coding genes, we provide the complete deletion primer set for deleting all noncoding RNAs in each genome. PRIMED have been tested for accuracy and are provided as downloadable Excel files.<br />
<br />
The publication describing the software and the primers it generated are described in a PLOS ONE [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116657 paper].</div>Jdemeter