Details of 2021 Reference Genome Annotation Update R64.3

From SGD-Wiki
Revision as of 17:06, 22 April 2021 by Stacia (talk | contribs)
Jump to: navigation, search

The S. cerevisiae strain S288C reference genome annotation was updated in its first major update since 2014. The new genome annotation is release R64.3.1, dated 2021-04-22. Note that the underlying genome sequence itself was not altered in any way.

This annotation update included (details in table below):

  • 5 new ncRNAs
  • 1 new recombination enhancer
  • 7 new ORFs
  • 1 new LTR
  • 2 new uORFs
  • 2 ORFs with shifted translation starts
  • 1 ORF with shifted translation stop plus new intron
  • Changed feature_type (and SO_term) for non-transcribed spacers
  • New systematic nomenclature system for entire annotated complement of ncRNAs


Chr Feature Description of change Reference
II YNCB0008W aka GAL10-ncRNA New ncRNA antisense to GAL10
  • coordinates 276805..280645
Houseley et al 2008 PMID:19061643,

Pinskaya et al 2009 PMID:19407817, Geisler et al 2012 PMID:22226051

II YNCB0014W aka TBRT/XUT_2F-154 New ncRNA antisense to TAT1
  • coordinates 376610..378633
Awasthi et al 2020 PMID:32081726
III RE/RE301 New recombination enhancer
  • coordinates 29108..29809
Wu and Haber 1996 PMID:8861911
V YELWdelta27 New Ty1 LTR PMID:29320491
V HPA3/YEL066W Moved translation start to Met19
old coordinates: 26667..27206
new coordinates: 26721..27206
PMID: 23775086
VII OTO1/YGR227C-A New ORF PMID: 25781884
VII ROK1/YGL171W Added two new uORFs
uORF1: 182286..182407
uORF2: 182291..182329
PMID:20969870
VIII YHR052C-B New ORF PMID:29897761
VIII YHR054C-B New ORF PMID:29897761
VIII SUT169/YNCH0011W New ncRNA PMID:19169243, PMID:22226051, PMID:27292640, PMID:28977638
X YJR107C-A New ORF PMID:26554900, PMID:29897761
X YJR012C Moved start to Met76
old coordinates: 459795..460418 Crick
new coordinates: 459795..460193 Crick
PMID:29632376
XI YKL104W-A New ORF PMID:29897761
XII YLR379W-A
XII NTS1-2, NTS2-1, NTS2-2 Change feature_type/SO_term from SO:0001637 rRNA_gene to SO:0000183 non_transcribed_region
XIII LDO45/YMR147W Shift stop to be same as LDO16/YMR148W, add intron
old coordinates 559199..559870
new coordinates 559199..559780, 560156..560812
PMID:29187527
XIII YMR008C-A
XIII YNCM0001W aka PHO84 lncRNA New ncRNA PMID:18022365
XIV LTO1/YNL260C Move start to Met37
old coords: 156859..157455 Crick
new coords: 156859..157347 Crick
PMID: 26182403
XVI YNCP0002W aka GAL4 lncRNA New ncRNA PMID: 22226051