Difference between revisions of "Commonly used strains"

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= Description of page =
 
This table describes some of the most commonly used yeast lab strains. Much of the information is taken from [http://dbb.urmc.rochester.edu/labs/sherman_f/StartedYeast.html F. Sherman (2002)] Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include
 
This table describes some of the most commonly used yeast lab strains. Much of the information is taken from [http://dbb.urmc.rochester.edu/labs/sherman_f/StartedYeast.html F. Sherman (2002)] Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include
  
Line 6: Line 7:
  
 
= Strain backgrounds =
 
= Strain backgrounds =
−
 
  
 
=S288C=
 
=S288C=
Line 15: Line 15:
 
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000050744 Mortimer and Johnston] (1986) Genetics 113:35-43.
 
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000050744 Mortimer and Johnston] (1986) Genetics 113:35-43.
  
−
'''Sources:'''[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204508 ATCC: 204508]
+
'''Sources:'''[http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204508 ATCC:204508]
 +
 
 +
==BY4743==
 +
'''Genotype:''' ''MAT''a/α ''his3''Δ''1/his3''Δ''1 leu2''Δ''0/leu2''Δ''0 lys2''Δ''0/LYS2 MET15/met15''Δ''0 ura3''Δ''0/ura3''Δ''0''
 +
 
 +
'''Notes:'''Strain used in the [http://www-sequence.stanford.edu/group/yeast_deletion_project/project_desc.html systematic deletion project], generated from a cross between BY4741 and BY4742, which are derived from S288C. See Brachmann et al. reference for details.
  
−
=FY1679=
+
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al.] (1998) Yeast 14:115-32.
−
'''Genotype:''' ''MAT''a/? ''ura3-52/ura3-52 trp1''?''63/TRP1 leu2''?'' 1/LEU2 his3''?''200/HIS3 GAL2/GAL''
+
 
 +
'''Sources:''' [http://www.openbiosystems.com/Query/?i=0&q=YSC1050|Open Biosystems:YSC1050]
 +
 
 +
==FY1679==
 +
'''Genotype:''' ''MAT''a/α ''ura3-52/ura3-52 trp1''Δ''63/TRP1 leu2''Δ''1/LEU2 his3''Δ''200/HIS3 GAL2/GAL''
  
 
'''Notes:'''  Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
 
'''Notes:'''  Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
Line 24: Line 33:
 
'''References:'''[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000047446 Winston et al.] (1995) Yeast 11:53-55.
 
'''References:'''[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000047446 Winston et al.] (1995) Yeast 11:53-55.
  
−
'''Sources:''' [http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/fy1679.html EUROSCARF: 10000D]
+
'''Sources:''' [http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/fy1679.html EUROSCARF:10000D]
 +
 
 +
==AB972==
 +
'''Genotype:''' ''MAT''&#x3B1;'' X2180-1B trp1<sub>0</sub> [rho <sup>0</sup>]''
 +
 
 +
'''Notes:''' Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-''trp1''.
 +
 
 +
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000057090 Olson MV et al.] (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.
 +
 
 +
'''Sources:''' [http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204511 ATCC:204511]
 +
 
 +
==A364A==
 +
'''Genotype:''' ''MAT''a'' ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup''
 +
 
 +
'''Notes:''' Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
 +
 
 +
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000079649 Hartwell] (1967) J. Bacteriol. 93:1662-1670.
 +
 
 +
'''Sources:''' [http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=208526 ATCC:208526]
 +
 
 +
==XJ24-24a==
 +
'''Genotype:''' ''MAT''a ''ho HMa HM&#x3B1; ade6 arg4-17 trp1-1 tyr7-1 MAL2''
 +
 
 +
'''Notes:''' Derived from, but not isogenic to S288C
 +
 
 +
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000055409 Strathern et al.] (1979) Cell 18:309-319
 +
 
 +
==DC5==
 +
'''Genotype:''' ''MAT''a'' leu2-3,112 his3-11,15 can1-11''
 +
 
 +
'''Notes:''' Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
 +
 
 +
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000054242 Broach et al.] (1979) Gene 8:121-133
 +
 
 +
==YNN216==
 +
'''Genotype:''' ''MAT''a/&#x3B1; ''ura3-52/ura3-52 lys2-801<sup>amber</sup>/lys2-801<sup>amber</sup> ade2-101<sup>ochre</sup>/ade2-101<sup>ochre</sup>''
 +
 
 +
'''Notes:''' Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).
 +
 
 +
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000042217 Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.
 +
==YPH499==
 +
'''Genotype:''' ''MAT''a ''ura3-52 lys2-801_amber ade2-101_ochre trp1-''&#x394;''63 his3-''&#x394;''200 leu2-''&#x394;''1''
 +
 
 +
'''Notes:''' Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note that ''trp1-''&#x394;''63'', unlike ''trp1-''&#x394;''1'', does not delete adjacent ''GAL3'' UAS sequence and retains homology to ''TRP1'' selectable marker. ''gal2-'', does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
 +
 
 +
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000042217 Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?pubmed=6092912 Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
 +
 
 +
'''Sources:''' [http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204679 ATCC:204679]
 +
 
 +
==YPH500==
 +
'''Genotype:''' ''MAT''&#x3B1; ''ura3-52 lys2-801_amber ade2-101_ochre trp1-''&#x394;''63 his3-''&#x394;''200 leu2-''&#x394;''1''
 +
 
 +
'''Notes:'''''MAT''&#x3B1; strain isogenic to [http://staff.yeastgenome.org/index.php/straintable#yph499 YPH499] except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
 +
 
 +
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000042217 Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?pubmed=6092912 Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
 +
 
 +
'''Sources:''' [http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204680 ATCC:204680]
 +
 
 +
==YPH501==
 +
'''Genotype:''' ''MAT''a/''MAT''&#x3B1; ''ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-''&#x394;''63/trp1-''&#x394;''63 his3-''&#x394;''200/his3-''&#x394;''200 leu2-''&#x394;''1/leu2-''&#x394;''1''
 +
 
 +
'''Notes:'''a/&#x3B1; diploid isogenic to [http://staff.yeastgenome.org/index.php/straintable#yph499 YPH499] and [http://staff.yeastgenome.org/index.php/straintable#yph500 YPH500]. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
 +
 
 +
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000042217 Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?pubmed=6092912 Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
 +
 
 +
'''Sources:''' [http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204681 ATCC:204681]
 +
 
 +
=Sigma 1278B=
 +
 
 +
'''Notes:'''Used in pseudohyphal growth studies. [http://staff.yeastgenome.org/community/sigma.html Detailed notes] about the sigma strains have been kindly provided by Cora Styles.
 +
 
 +
=SK1=
 +
'''Genotype:''' ''MAT''a/&#x3B1;'' HO gal2 cup<sup>S</sup> can1<sup>R</sup>''
  
−
=BY4743=
+
'''Notes:''' Commonly used for studying sporulation or meiosis. Canavanine-resistant derivative
−
'''Genotype:''' ''MAT''a/? ''his3''?''1/his3''?''1 leu2''?''0/leu2''?''0 lys2''?''0/LYS2 MET15/met15''?''0 ura3''?''0/ura3''?''0''
 
  
−
'''Notes:'''Strain used in the [http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www-sequence.stanford.edu/group/yeast_deletion_project/project_desc.html systematic deletion project], generated from a cross between BY4741 and BY4742, which are derived from S288C. See Brachmann et al. reference for details.
+
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000079650 Kane SM and Roth J.] (1974) Bacteriol. 118: 8-14
  
−
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al.] (1998) Yeast 14:115-32.
+
'''Sources:''' [http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204722 ATCC:204722]
 +
 
 +
=CEN.PK (aka CEN.PK2)=
 +
'''Genotype:''' ''MAT''a/&#x3B1;'' ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 ''&#x394;''1/his3 ''&#x394;''1 MAL2-8C/MAL2-8C SUC2/SUC2''
 +
 
 +
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000079648 van Dijken et al.] (2000) Enzyme Microb Technol 26:706-714
  
−
'''Sources:''' [http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.openbiosystems.com/ Open Biosystems: YSC1050]
+
'''Sources:''' [http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/cen.html EUROSCARF:30000D]
  
 
=W303=
 
=W303=
−
'''Genotype:''' ''MAT''a/''MAT''? {''leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15''} [''phi<sup>+</sup>'']
+
'''Genotype:''' ''MAT''a/''MAT''&#x3B1; {''leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15''} [''phi<sup>+</sup>'']
  
 
'''Notes:''' W303 also contains a ''bud4'' mutation that causes
 
'''Notes:''' W303 also contains a ''bud4'' mutation that causes
Line 45: Line 130:
 
'''References:''' W303 constructed by Rodney Rothstein (''see [http://wiki.yeastgenome.org/index.php/CommunityW303.html detailed notes] from RR'').<br />''bud4'' info: Voth, W.P. et al. (2005) Eukaryotic Cell, in press. <br />''rad5-535'' info: Fan et al. (1996) Genetics 142:749
 
'''References:''' W303 constructed by Rodney Rothstein (''see [http://wiki.yeastgenome.org/index.php/CommunityW303.html detailed notes] from RR'').<br />''bud4'' info: Voth, W.P. et al. (2005) Eukaryotic Cell, in press. <br />''rad5-535'' info: Fan et al. (1996) Genetics 142:749
  
−
'''Sources:''' [http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.openbiosystems.com/Genomics/Model Organism Resources/Yeast Resources/Yeast Knockout Strains/ Open Biosystems: YSC1058]
+
'''Sources:''' [http://www.openbiosystems.com/Query/?i=0&q=YSC1058|Open Biosystems:YSC1058]
 +
 
 +
==W303-1A==
 +
'''Genotype:''' ''MAT''a {''leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15''}
 +
 
 +
'''Notes:''' W303-1A possesses a ''ybp1-1'' mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.
 +
 
 +
'''References:''' W303 constructed by Rodney Rothstein (''see [http://wiki.yeastgenome.org/index.php/CommunityW303.html detailed notes] from RR'').<br />''ybp1-1'' info: [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000073844 Veal et al.] (2003) J. Biol. Chem. 278:30896-904.<br />
 +
 
 +
'''Sources:''' [http://www.openbiosystems.com/Query/?i=0&q=YSC1058|Open Biosystems:YSC1058]
 +
 
 +
==W303-1B==
 +
'''Genotype:''' ''MAT''&#x3B1; {''leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15''}
 +
 
 +
'''References:''' W303 constructed by Rodney Rothstein (''see [http://wiki.yeastgenome.org/index.php/CommunityW303.html detailed notes] from RR'').
 +
 
 +
'''Sources:''' [http://www.openbiosystems.com/Query/?i=0&q=YSC1058|Open Biosystems:YSC1058]
 +
 
 +
=X2180-1A=
 +
'''Genotype:''' ''MAT''a'' SUC2 mal mel gal2 CUP1''
 +
 
 +
'''References:'''  Robert Mortimer, personal comm.
 +
 
 +
'''Sources:''' [http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204504 ATCC:204504]
 +
 
 +
=D273-10B=
 +
'''Genotype:''' ''MAT''&#x3B1; ''mal''
 +
 
 +
'''Notes:''' Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.
 +
 
 +
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000080158 Sherman, F.] (1963) Genetics 48:375-385.
 +
 
 +
'''Sources:''' [http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=24657 ATCC:24657]
  
−
{| border="3"
+
=FL100=
−
|- align="center"
+
'''Genotype:''' ''MAT''a
−
! width="5%" | Strain
 
−
! width="30%" | Genotype
 
−
! width="30%" | Notes
 
−
! width="30%" | References
 
−
! width="5%" | ATCC/Open Biosystems/EUROSCARF
 
−
|-
 
−
|<div id=S288C>S288C
 
−
| ''MAT''α ''SUC2 gal2 mal mel flo1 flo8-1 hap1''
 
−
|
 
−
Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of [http://db.yeastgenome.org/cgi-bin/locus.pl?dbid=S000004246 ''HAP1''], it is not a good strain for mitochondrial studies. S288C strains are ''gal''2- and they do not use galactose anaerobically.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000050744 Mortimer and Johnston] (1986) Genetics 113:35-43.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204508 ATCC: 204508]
 
−
|-
 
−
|<div id=FY1679>FY1679
 
−
| ''MAT''a/α ''ura3-52/ura3-52 trp1''Δ''63/TRP1 leu2''Δ'' 1/LEU2 his3''Δ''200/HIS3 GAL2/GAL''
 
−
| Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000047446 Winston et al.] (1995) Yeast 11:53-55.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/fy1679.html EUROSCARF: 10000D]
 
−
|-
 
−
|<div id=BY4743>BY4743
 
−
| ''MAT''a/α ''his3''Δ''1/his3''Δ''1 leu2''Δ''0/leu2''Δ''0 lys2''Δ''0/LYS2 MET15/met15''Δ''0 ura3''Δ''0/ura3''Δ''0''
 
−
|
 
−
Strain used in the [http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www-sequence.stanford.edu/group/yeast_deletion_project/project_desc.html systematic deletion project], generated from a cross between BY4741 and BY4742, which are derived from S288C. See Brachmann et al. reference for details.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al.] (1998) Yeast 14:115-32.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.openbiosystems.com/ Open Biosystems: YSC1050]
 
−
|-
 
−
|<div id=W303>W303
 
−
| ''MAT''a/''MAT''α {''leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15''} [''phi<sup>+</sup>'']
 
−
| W303 also contains a ''bud4'' mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the ''rad5-535'' allele.
 
−
| W303 constructed by Rodney Rothstein (''see [http://wiki.yeastgenome.org/index.php/CommunityW303.html detailed notes] from RR'').<br />''bud4'' info: Voth, W.P. et al. (2005) Eukaryotic Cell, in press. <br />''rad5-535'' info: Fan et al. (1996) Genetics 142:749
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.openbiosystems.com/Genomics/Model Organism Resources/Yeast Resources/Yeast Knockout Strains/ Open Biosystems: YSC1058]
 
−
|-
 
−
|<div id=W303-1A>W303-1A
 
−
| ''MAT''a {''leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15''}
 
−
| W303-1A possesses a ''ybp1-1'' mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.
 
−
|
 
−
W303 constructed by Rodney Rothstein (''see [http://wiki.yeastgenome.org/index.php/CommunityW303.html detailed notes] from RR'').<br />''ybp1-1'' info: [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000073844 Veal et al.] (2003) J. Biol. Chem. 278:30896-904.<br />
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.openbiosystems.com/Genomics/Model Organism Resources/Yeast Resources/Yeast Knockout Strains/ Open Biosystems: YSC1058]
 
−
|-
 
−
|<div id=W303-1B>W303-1B
 
−
| ''MAT''α {''leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15''}
 
−
|
 
−
| W303 constructed by Rodney Rothstein (''see [http://wiki.yeastgenome.org/index.php/CommunityW303.html detailed notes] from RR'').
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.openbiosystems.com/Genomics/Model Organism Resources/Yeast Resources/Yeast Knockout Strains/ Open Biosystems: YSC1058]
 
−
|-
 
−
|<div id=X2180-1A>X2180-1A
 
−
| ''MAT''a'' SUC2 mal mel gal2 CUP1''
 
−
|
 
−
| Robert Mortimer, personal comm.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204504 ATCC: 204504]
 
−
|-
 
−
|<div id=AB972>AB972
 
−
| ''MAT''α'' X2180-1B trp1<sub>0</sub> [rho <sup>0</sup>]''
 
−
| Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-''trp1''.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000057090 Olson MV et al.] (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204511 ATCC: 204511]
 
−
|-
 
−
|<div id=A364A>A364A
 
−
| ''MAT''a'' ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup''
 
−
| Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000079649 Hartwell] (1967) J. Bacteriol. 93:1662-1670.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=208526 ATCC: 208526]
 
−
|-
 
−
|<div id="Sigma_1278B">Sigma 1278B
 
−
|
 
−
|
 
−
Used in pseudohyphal growth studies. [http://staff.yeastgenome.org/community/sigma.html Detailed notes] about the sigma strains have been kindly provided by Cora Styles.
 
−
|
 
−
|
 
−
|-
 
−
|<div id=SK1>SK1 (canavanine-resistant derivative)
 
−
| ''MAT''a/α'' HO gal2 cup<sup>S</sup> can1<sup>R</sup>''
 
−
| Commonly used for studying sporulation or meiosis
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000079650 Kane SM and Roth J.] (1974) Bacteriol. 118: 8-14
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204722 ATCC: 204722]
 
−
|-
 
−
|<div id=CEN.PK2>CEN.PK (aka CEN.PK2)
 
−
| ''MAT''a/α'' ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 ''Δ''1/his3 ''Δ''1 MAL2-8C/MAL2-8C SUC2/SUC2''
 
−
|
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000079648 van Dijken et al.] (2000) Enzyme Microb Technol 26:706-714
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/cen.html EUROSCARF: 30000D]
 
−
|-
 
−
|<div id=XJ24-24a>XJ24-24a
 
−
| ''MAT''a ''ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2''
 
−
| Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000055409 Strathern et al.] (1979) Cell 18:309-319
 
−
|
 
−
|-
 
−
|<div id=DC5>DC5
 
−
| ''MAT''a'' leu2-3,112 his3-11,15 can1-11''
 
−
| Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000054242 Broach et al.] (1979) Gene 8:121-133
 
−
|
 
−
|-
 
−
|<div id=YNN216>YNN216
 
−
| ''MAT''a/α ''ura3-52/ura3-52 lys2-801<sup>amber</sup>/lys2-801<sup>amber</sup> ade2-101<sup>ochre</sup>/ade2-101<sup>ochre</sup>''
 
−
| Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000042217 Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.
 
−
|
 
−
|-
 
−
|<div id=D273-10B>D273-10B
 
−
| ''MAT''α ''mal''
 
−
| Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000080158 Sherman, F.] (1963) Genetics 48:375-385.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=24657 ATCC: 24657]
 
−
|-
 
−
|<div id=FL100>FL100
 
−
| ''MAT''a
 
−
|
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000065623 Lacroute, F.] (1968) J. Bacteriol. 95:824-832.
 
−
|
 
−
|-
 
−
|<div id=YPH499>YPH499
 
−
| ''MAT''a ''ura3-52 lys2-801_amber ade2-101_ochre trp1-''Δ''63 his3-''Δ''200 leu2-''Δ''1''
 
−
| Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note that ''trp1-''Δ''63'', unlike ''trp1-''Δ''1'', does not delete adjacent ''GAL3'' UAS sequence and retains homology to ''TRP1'' selectable marker. ''gal2-'', does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000042217 Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?pubmed=6092912 Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204679 ATCC: 204679]
 
−
|-
 
−
|<div id=YPH500>YPH500
 
−
| ''MAT''α ''ura3-52 lys2-801_amber ade2-101_ochre trp1-''Δ''63 his3-''Δ''200 leu2-''Δ''1''
 
−
|
 
−
''MAT''α strain isogenic to [http://staff.yeastgenome.org/index.php/straintable#yph499 YPH499] except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000042217 Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?pubmed=6092912 Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204680 ATCC:204680]
 
−
|-
 
−
|<div id=YPH501>YPH501
 
−
| ''MAT''a/''MAT''α ''ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-''Δ''63/trp1-''Δ''63 his3-''Δ''200/his3-''Δ''200 leu2-''Δ''1/leu2-''Δ''1''
 
−
|
 
−
a/α diploid isogenic to [http://staff.yeastgenome.org/index.php/straintable#yph499 YPH499] and [http://staff.yeastgenome.org/index.php/straintable#yph500 YPH500]. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski RS and Hieter P] (1989) Genetics 122:19-27.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000042217 Sobel and Wolin] (1999) Mol. Biol. Cell 10:3849-3862.<br />[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?pubmed=6092912 Johnston M and Davis RW] (1984) Mol Cell Biol 4(8):1440-8.
 
−
|
 
−
[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204681 ATCC:204681]
 
−
|}
 
  
−
{|
+
'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000065623 Lacroute, F.] (1968) J. Bacteriol. 95:824-832.
−
|
 
−
| ''''
 
−
|
 
−
|
 
−
|
 
−
|}
 

Revision as of 17:04, 20 July 2007

Description of page

This table describes some of the most commonly used yeast lab strains. Much of the information is taken from F. Sherman (2002) Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include

  • Mortimer and Johnston (1986) Genetics 113:35-43 - thoroughly describes the genealogy of strain S288C
  • van Dijken et al. (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly used lab strains
  • Winzeler et al. (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identity between common lab strains

Strain backgrounds

S288C

Genotype: MATα SUC2 gal2 mal mel flo1 flo8-1 hap1

Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. S288C strains are gal2- and they do not use galactose anaerobically.

References: Mortimer and Johnston (1986) Genetics 113:35-43.

Sources:ATCC:204508

BY4743

Genotype: MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 MET15/met15Δ0 ura3Δ0/ura3Δ0

Notes:Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. See Brachmann et al. reference for details.

References: Brachmann et al. (1998) Yeast 14:115-32.

Sources: Biosystems:YSC1050

FY1679

Genotype: MATa/α ura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 GAL2/GAL

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References:Winston et al. (1995) Yeast 11:53-55.

Sources: EUROSCARF:10000D

AB972

Genotype: MATα X2180-1B trp10 [rho 0]

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.

References: Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.

Sources: ATCC:204511

A364A

Genotype: MATa ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References: Hartwell (1967) J. Bacteriol. 93:1662-1670.

Sources: ATCC:208526

XJ24-24a

Genotype: MATa ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2

Notes: Derived from, but not isogenic to S288C

References: Strathern et al. (1979) Cell 18:309-319

DC5

Genotype: MATa leu2-3,112 his3-11,15 can1-11

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References: Broach et al. (1979) Gene 8:121-133

YNN216

Genotype: MATa/α ura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre

Notes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.

YPH499

Genotype: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note that trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker. gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

Sources: ATCC:204679

YPH500

Genotype: MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes:MATα strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

Sources: ATCC:204680

YPH501

Genotype: MATa/MATα ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-Δ63/trp1-Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1

Notes:a/α diploid isogenic to YPH499 and YPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

Sources: ATCC:204681

Sigma 1278B

Notes:Used in pseudohyphal growth studies. Detailed notes about the sigma strains have been kindly provided by Cora Styles.

SK1

Genotype: MATa/α HO gal2 cupS can1R

Notes: Commonly used for studying sporulation or meiosis. Canavanine-resistant derivative

References: Kane SM and Roth J. (1974) Bacteriol. 118: 8-14

Sources: ATCC:204722

CEN.PK (aka CEN.PK2)

Genotype: MATa/α ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 Δ1/his3 Δ1 MAL2-8C/MAL2-8C SUC2/SUC2

References: van Dijken et al. (2000) Enzyme Microb Technol 26:706-714

Sources: EUROSCARF:30000D

W303

Genotype: MATa/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+]

Notes: W303 also contains a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele.

References: W303 constructed by Rodney Rothstein (see detailed notes from RR).
bud4 info: Voth, W.P. et al. (2005) Eukaryotic Cell, in press.
rad5-535 info: Fan et al. (1996) Genetics 142:749

Sources: Biosystems:YSC1058

W303-1A

Genotype: MATa {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}

Notes: W303-1A possesses a ybp1-1 mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.

References: W303 constructed by Rodney Rothstein (see detailed notes from RR).
ybp1-1 info: Veal et al. (2003) J. Biol. Chem. 278:30896-904.

Sources: Biosystems:YSC1058

W303-1B

Genotype: MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}

References: W303 constructed by Rodney Rothstein (see detailed notes from RR).

Sources: Biosystems:YSC1058

X2180-1A

Genotype: MATa SUC2 mal mel gal2 CUP1

References: Robert Mortimer, personal comm.

Sources: ATCC:204504

D273-10B

Genotype: MATα mal

Notes: Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.

References: Sherman, F. (1963) Genetics 48:375-385.

Sources: ATCC:24657

FL100

Genotype: MATa

References: Lacroute, F. (1968) J. Bacteriol. 95:824-832.

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