Commonly used auxotrophic markers

This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in Brachmann et al. (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you have additions or modifications you would like to suggest.

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Allele	Deleted ORF?	Reverts?	Notes	Molecular Descriptiona	Reference
ade1-14	no	yes	red colonies	TGG-to-TGA nonsense change at codon 244; GGA-to-GAA missense change at codon 185.	Nakayashiki et al. 2001
ade2-1	no	yes	ochre mutation	TTA-to-TTG silent change at codon 9, GAA-to-TAA ochre nonsense change at codon 64, AGA-to-GGA missense change at codon 101, GTT-to-GTC silent change at codon 124, ACG-to-ACA silent change at codon 539	Rodney Rothstein, Personal communication to SGD
ade2-101	no	yes	ochre mutation, red colonies	G to T transversion at nucleotide 190, changing codon 64 from a Glu to a Stop	Gai and Voytas, 2005
ade2-BglII	no	no	red colonies	frameshift (BglII site filled in at position 592)	Engebrecht and Roeder 1990
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^aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.

^bAll trp- strains are cold sensitive (Singh and Manney 1974).