Difference between revisions of "Commonly used auxotrophic markers"

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(Table edited by Maria via TableEdit)
(Table edited by Maria via TableEdit)
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[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000060944 Nakayashiki et al. 2001]  
 
[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000060944 Nakayashiki et al. 2001]  
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[http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=ade2 ade2-1]
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no
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yes
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ochre mutation
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TTA-to-TTG silent change at codon 9,
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GAA-to-TAA ochre nonsense change at codon 64,
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AGA-to-GGA missense change at codon 101,
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GTT-to-GTC silent change at codon 124,
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ACG-to-ACA silent change at codon 539
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Rodney Rothstein, [http://wiki.yeastgenome.org/index.php/CommunityW303.html Personal communication to SGD]
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[http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=ade2 ade2-101]
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no
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yes
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ochre mutation, red colonies
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G to T transversion at nucleotide 190, changing codon 64 from a Glu to a Stop
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[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000087056 Gai and Voytas, 2005]
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[http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=ade2 ade2-BglII]
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no
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no
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red colonies
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frameshift (BglII site filled in at position 592)
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[http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000049198 Engebrecht and Roeder 1990]
  
 
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Revision as of 11:10, 8 March 2012

This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in Brachmann et al. (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you have additions or modifications you would like to suggest.

   <protect>
Allele Deleted ORF? Reverts? Notes Molecular Descriptiona Reference

ade1-14

no

yes

red colonies

TGG-to-TGA nonsense change at codon 244; GGA-to-GAA missense change at codon 185.

Nakayashiki et al. 2001

ade2-1

no

yes

ochre mutation

TTA-to-TTG silent change at codon 9, GAA-to-TAA ochre nonsense change at codon 64, AGA-to-GGA missense change at codon 101, GTT-to-GTC silent change at codon 124, ACG-to-ACA silent change at codon 539

Rodney Rothstein, Personal communication to SGD

ade2-101

no

yes

ochre mutation, red colonies

G to T transversion at nucleotide 190, changing codon 64 from a Glu to a Stop

Gai and Voytas, 2005

ade2-BglII

no

no

red colonies

frameshift (BglII site filled in at position 592)

Engebrecht and Roeder 1990

</protect>



aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.

bAll trp- strains are cold sensitive (Singh and Manney 1974).