Difference between revisions of "Commonly used auxotrophic markers"
(Table edited by Maria via TableEdit) |
|||
| Line 10: | Line 10: | ||
{| id="W4f58df1c2e2f4" class=" tableEdit " | {| id="W4f58df1c2e2f4" class=" tableEdit " | ||
|- | |- | ||
| − | !|Allele!!Deleted ORF?!!Reverts?!!Molecular Description<sup>a</sup>!!Reference | + | !|Allele!!Deleted ORF?!!Reverts?!!Notes!!Molecular Description<sup>a</sup>!!Reference |
| + | |- | ||
| + | | | ||
| + | [http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=ade1 ade1-14] | ||
| + | | | ||
| + | no | ||
| + | | | ||
| + | yes | ||
| + | | | ||
| + | red colonies | ||
| + | | | ||
| + | TGG-to-TGA nonsense change at codon 244; | ||
| + | GGA-to-GAA missense change at codon 185. | ||
| + | | | ||
| + | [http://www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000060944 Nakayashiki et al. 2001] | ||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
| − | |<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d001ef25e9c0a93e0c26b710b67e1344.28469.W4f58df1c2e2f4&page=28469&pagename={{FULLPAGENAMEE}}&type=0&template= edit table]</span> || || || || | + | |<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=d001ef25e9c0a93e0c26b710b67e1344.28469.W4f58df1c2e2f4&page=28469&pagename={{FULLPAGENAMEE}}&type=0&template= edit table]</span> || || || || || |
|} | |} | ||
<!--box uid=d001ef25e9c0a93e0c26b710b67e1344.28469.W4f58df1c2e2f4--></protect> | <!--box uid=d001ef25e9c0a93e0c26b710b67e1344.28469.W4f58df1c2e2f4--></protect> | ||
Revision as of 11:02, 8 March 2012
This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in Brachmann et al. (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you have additions or modifications you would like to suggest.
<protect>
| Allele | Deleted ORF? | Reverts? | Notes | Molecular Descriptiona | Reference |
|---|---|---|---|---|---|
|
no |
yes |
red colonies |
TGG-to-TGA nonsense change at codon 244; GGA-to-GAA missense change at codon 185. |
||
| edit table |
</protect>
aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.
bAll trp- strains are cold sensitive (Singh and Manney 1974).
