YOR027W
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| Systematic name | YOR027W |
| Gene name | STI1 |
| Aliases | |
| Feature type | ORF, Verified |
| Coordinates | Chr XV:381053..382822 |
| Primary SGDID | S000005553 |
Description of YOR027W: Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop[1][2][3][4][5]
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Community Commentary
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Interactions
Regulatory
Regulatory interaction with Ssa1
Binding of Sti1 to Ssa1 activates the ATPase of Ssa1 by a factor of about 200, which is in contrast to the behaviour of Hop in the mammalian Hsp70 system. Analysis of the underlying activation mechanism revealed that ATP hydrolysis is rate limiting in the Ssa1 ATPase cycle and that this step is accelerated by Sti1. [5] [6]
Two Hybrid
Two Hybrid interaction with Ssa1
With a two hybrid approach and coimmunoprecipitations, we show that Sti1 specifically interacts with the Ssa group of the cytosolic yeast Hsp70 proteins and not with the Ssb, Sse and Ssz group. [5] [6]
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References
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- ↑ Chang HC, et al. (1997) In vivo analysis of the Hsp90 cochaperone Sti1 (p60). Mol Cell Biol 17(1):318-25 SGD PMID 8972212
- ↑ Li J, et al. (2011) Mixed Hsp90-cochaperone complexes are important for the progression of the reaction cycle. Nat Struct Mol Biol 18(1):61-6 SGD PMID 21170051
- ↑ Nicolet CM and Craig EA (1989) Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae. Mol Cell Biol 9(9):3638-46 SGD PMID 2674681
- ↑ Richter K, et al. (2003) Sti1 is a non-competitive inhibitor of the Hsp90 ATPase. Binding prevents the N-terminal dimerization reaction during the atpase cycle. J Biol Chem 278(12):10328-33 SGD PMID 12525481
- ↑ 5.0 5.1 5.2 Wegele H, et al. (2003) Sti1 is a novel activator of the Ssa proteins. J Biol Chem 278(28):25970-6
SGD PMID 12716905 Cite error: Invalid
<ref>tag; name "S000073412" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000073412" defined multiple times with different content - ↑ 6.0 6.1 submitted by Dr. Harald Wegele on 2003-09-03
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