YBL086C

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Systematic name YBL086C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr II:62602..61202
Primary SGDID S000000182


Description of YBL086C: Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery[1]




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Community Commentary

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Growth Curve

YBL086C YPD.png

In a BY4735 background, knocking out YBL086C seems to have little or no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YAL061 knock-out strain's doubling time was 495 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

megan21.png

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 743.32 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

The protocol can be found at

Caffeine

yBL086cUV Exposure.png

This bar graph Shows that compared to a BY4735 background, Knocking out yBL086c seems to make the yeast more sensitive to UV light exposure.


Cycloheximides effect on YBL086C

Cycloheximide 3.png

As we can see form the results Tests 2-4 succeeded on cycloheximide and Test 1 was effected. Growth Models 1-3 are the Yeast strands not under distress.


Effect of 3% DMSO on YBL086C

Effect of 3% DMSO on YBL086C.png

The graph above shows the effect of 3% DMSO on YBL086C. From the data represented the average doubling rate for the YBL086C knockout strain without DMSO was 617 minutes. When DMSO is added the average doubling time increased to 976 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YBL086C with DMSO we can see that without the YBL086C gene the yeast was able to grow more quickly under DMSO induced stress.


Ethanol Tolerance for YBL086C

YBL086C ethonal.png

This graph shows the growth curve of ethanol tolerance in YBL086C. The unstressed lines represent YBL086C that was not treated with ethanol. If you examine the graph you will notice that the strands that were treated with ethanol grew more than the ones that weren't. The average doubling time for the stressed yeast was 1053.88 minutes. The average doubling time for the unstressed yeast was 971.64 minutes. The results show that YAL061 in ethanol leads to an increase in growth.

1% Formamide Yeast Torture[[1]]

image 2.png

This graph shows the growth curve of the knockout yeast strain YBL086L and wild type BY4735 in 1% formamide in 3 trials. The average calculated doubling time for YBL086L with 1% formamide was 4,728.61 minutes, while the unstressed strain was 4,582.43 minutes. This concluded that the stressed out strain is slightly sensitive to 1% formamide, and grows at a slower pace in this environment. Our stressed wild types doubling time was 4,927.15 minutes, and the unstressed was 3,373.54 minutes. Again, proving that the addition of 1% formamide constrains growth.


Hydroxyurea

AvaYBL086C.png The averaging doubling time for YBL086C is 809.15 minutes. The averaging doubling time for the unstressed YBL086C is 387.58 minutes



Prednisone Effects on YBL086C

YBL086C.png

The graph above shows how dilutions P2 (30 µg/ml) and E2 (10% ethanol solution) impacted the growth rate of the knockout yeast strain YBL086C over 24 hours. The concentration did not double for test 3 of P2 for this experiment, so by taking only the first two test results, the doubling rate averages to around 1017.5 minutes for only ethanol. As for the Prednisone with ethanol, the doubling rate averages to around 907.5 minutes. Overall, ethanol decreased the rate of growth by almost 110 minutes. These results conclude that Prednisone promotes the growth of yeast cells without the gene YBL086C.

Zone of Inhibition of 30% Hydrogen Peroxide on YBL086C

JJ4.PNG JaredJ4.PNG The bar graph represents the zone of inhibition of the knockout strain versus the wild type. Our data collected shows that the knockout strain saw a smaller zone of inhibition (cells were not able to grow there). Using these observations we can conclude that the strain YBL086C can withstand more stress from the oxidative properties of 30 percent hydrogen peroxide. For the strain YBL086C our plate values were as follows (in cm) 2, 2 and 2 compared to our wild type plate which stayed constant 2.1, 2.1 and 2.2 cm <protect>

Growth in Proline and Ammonium Sulfate

KO2.png

The YBL086C strand grew about the same doubling rate in both proline and ammonium sulfate. The average doubling time in proline media was 41.1 minutes and in ammonium sulfate it was 40.13 minutes. So technically, this strand did grow faster in ammonium sulfate. The wild type had the faster average doubling time in ammonium sulfate media at a time of 46.5 minutes. In proline, the average doubling rate was 49.3 minutes.

Caption

The Wild Type BY4735 has data located at the Nitrogen Starvation Protocol.

Nitrogen starvation

References

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  1. ↑ Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095

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