UW-Stout/Saline SP23

Saltwater Torture

Pilot experiment

Introduction: This experiment is testing how much salt will stress out the yeast without killing them. Our goal of this experiment is to see how much salt is needed to allow the yeast to grow while altering their growth curve without killing them. In this pilot experiment we tested wild yeast strains against different molarities of salt water. Looking at the growth curve data will help us determine which molarity of salt water stressed out the yeast without killing them.

Materials:

• H2O
• NaCl (table salt)
• 12 2 ml centrifuge tubes
• Yeast cells

Procedure:

1. Calculate the dilution from 4 M saltwater into the varying amounts below. (see link below)
   1. concentration table
2. Make these solutions in each of the 12 2 ml centrifuge tubes
3. Micropipette 50 ul of yeast cells into 12 different wells
4. Micropipette 50 ul of the differing saltwater solutions into each well
5. Put into plate reader for 24-48 hours