YKL162C

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Revision as of 11:44, 2 May 2023 by Teagueb (talk | contribs) (Heat Shock)
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Systematic name YKL162C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr XI:148838..147630
Primary SGDID S000001645


Description of YKL162C: Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion[1]




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Community Commentary

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Isopropanol

UW-Stout/Isopropanol_SP23

Picture12.jpg

Analysis

These results were what was expected and all 3 were consistent throughout the trials. Although there was less growth than the control group, it could mean that the cell is more sensitive to the isopropanol with the gene knocked out. In the third trial there was a little bit of spiking towards the end around 15:20-18:00 but other than that it was a good growth curve.

Heat Shock

YKL162 Yeast Colonies at 40 degrees Celsius
Time (sec) Colonies
0 50
15 41
45 25
75 24
105 13
135 25
Wild Type Yeast Colonies at 40 degrees Celsius
Time (sec) Colonies
0 24
15 20
45 17
75 16
105 18
135 14

DKAtrial3yeast.jpg


KAD YKL162C.jpg The general trend of our data yielded a negative relationship between time of heat shock. About half of the yeast cells died off in a matter of 135 seconds at 40 degrees Celsius. It can be inferred that with a longer amount of time we the yeast cells could continue being killed off until all of them died.

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Bases

References

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  1. Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095

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