YER076C
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| Systematic name | YER076C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr V:313498..312590 |
| Primary SGDID | S000000878 |
Description of YER076C: Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization[1][2][3]
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Contents
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Ethanol
YER076C had the most cell growth. The doubling time was 71.60. Ethanol didn't affect the growth to much.
Caffeine
Interpretation
The average doubling time for the YER076C (C4) gene in standard conditions was 87.5 minutes. Under caffeine stress, the average doubling time was 139.5 minutes. That is a 59.4% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.
Hydrogen Peroxide
This is the link to the protocol page: UW-Stout/Hydrogen Peroxide FA22.
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References
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- Jump up ↑ Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54 SGD PMID 16823961
- Jump up ↑ Sickmann A, et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12 SGD PMID 14576278
- Jump up ↑ Terashima H, et al. (2002) Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae. Curr Genet 40(5):311-6 SGD PMID 11935221
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UW Stout/FA 2022 Glycerol Media
Introduction
Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.
Results
The following doubling times were calculated:
- Glycerol Trial 1: NA
- Negative Control Trial 1: 188 min
- Glycerol Trial 2: 144 min
- Negative Control Trial 2: 192 min
- Glycerol Trial 3:2531 min
- Negative Control Trial 3: 212 min
Analysis/Conclusion
Our results for our glycerol media is not consistent, the first trial was messed up, and the second and third trial have a large difference. Trials 2 & 3 bring up some issues because trial 2's time is lower than the negative control which would mean that this knockout gene strain of yeast would work better in the glycerol media than the glucose media, but our time for trial 3 is much larger than the negative control, and because our first trial was inconclusive we would need to run another trial to figure out whether or not this strain would truly live better in the glycerol media or not.
