UW-Stout/Hydrogen Peroxide SP22
Contents
Introduction
Hydrogen Peroxide (H2O2) is a very strong oxidizer and antiseptic that is often used in dilute forms. The overall compound is found in biological systems but is in short lived reactions due to it's high toxicity over time. The goal of our experiment was to test how multiple yeast strains with knockout genes would respond in the presence of varying concentrations of hydrogen peroxide.
Materials
- Hydrogen Peroxide Solution 3% Concentration
- Distilled water for dilutions
- Wild Type and Knockout Yeast Strains
Equipment
- P-200 Micropipette
- P-20 Micropipette
- P-10 Micropipette
- Centrifuge Tubes
- Yeast Test Well
Calibration Protocol
H2O2 Dilutions
- Vortex Yeast Strains
- Create 50ul Hydrogen Peroxide Dilutions As Required
- Mix Dilutions With 50ul yeast cultures
- Put Individual Wells to Growth For 24 Hours
Trial 1 Percent Series:3%, 1%, 0.3%, 0.1%, 0.03%, 0.01%
Trial 2 Percent Series: 3%, 2%, 1.33%, 0.88%, 0.59%, 0.40%
