UW-Stout/Hydrogen Peroxide SP22

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Introduction

Hydrogen Peroxide (H2O2) is a very strong oxidizer and antiseptic that is often used in dilute forms. The overall compound is found in biological systems but is in short lived reactions due to it's high toxicity over time. The goal of our experiment was to test how multiple yeast strains with knockout genes would respond in the presence of varying concentrations of hydrogen peroxide.


Materials

  • Hydrogen Peroxide Solution 3% Concentration
  • Distilled water for dilutions
  • Wild Type and Knockout Yeast Strains


Equipment

  • P-200 Micropipette
  • P-20 Micropipette
  • P-10 Micropipette
  • Centrifuge Tubes
  • Yeast Test Well

Calibration Protocol

H2O2 Percent Pilot (c2) (v2/c1) Trial 1 Percent Series: 3%, 1%, 0.3%, 0.1%, 0.03%, 0.01%

Peroxide left, water right. 50 + 0 16.67 + 33.33 5.5 + 44.50 1.67 + 48. 33 0.5 + 49.50 1.7 + 48.83 = 0.1 1.08 + 48.92 = 0.065

Trial 2 Percent Series; 3%, 2%, 1.33%, 0.88%, 0.59%, 0.40%

Peroxide left, water right. 33.33 + 16.67 22.2 + 27.8 14.8 + 35.2 9.8 + 40.2 6.7 + 43.3

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