UW-Stout/Copper FA21
Contents
Materials & Equipment
- CuSO4
- Sterile Water
- 1000ul Pipette
- 50ul Pipette
- Centrifuge Tubes
- Wild Type Yeast Cells
- Yeast Cells with Your Favorite Gene removed.
- Vortex
- Disposable Pipette Tips
- Plate Reader
- Wells
Personal Protective Equipment
- Latex Gloves
- Eye Pro
- Lab Coat
Wild Type Protocol
- Vortex Wild type Yeast Solutions
- Create Solutions with Copper Sulfate and Sterile water at the following Concentrations.
- 20mM (Solution) Copper Sulfate
- 10mM Copper Sulfate
- 5.0mM Copper Sulfate
- 2.0mM Copper Sulfate
- 1.0mM Copper Sulfate
- 0.5mM Copper Sulfate
- 0.2mM Copper Sulfate
- 0.1mM Copper Sulfate
- Combine 50ul of the Copper Sulfate solution and 50ul of the wild type yeast cells and put them in eight different wells.
- Take the wells that are prepared with the yeast and the Copper Sulfate and put it in a plate reader and leave it there for 24 hours.