Details of 2021 Reference Genome Annotation Update R64.3
The S. cerevisiae strain S288C reference genome annotation was updated in its first major update since 2014. The new genome annotation is release R64.3.1, dated 2021-04-22. Note that the underlying genome sequence itself was not altered in any way.
This annotation update included (details in table below):
- 5 new ncRNAs
- 1 new recombination enhancer
- 7 new ORFs
- 1 new LTR
- 2 new uORFs
- 2 ORFs with shifted translation starts
- 1 ORF with shifted translation stop plus new intron
- Changed feature_type (and SO_term) for non-transcribed spacers
- New systematic nomenclature system for entire annotated complement of ncRNAs
| Chr | Feature | Description of change | Reference |
|---|---|---|---|
| II | YNCB0008W aka GAL10-ncRNA | New ncRNA
coordinates 276805..280645 |
https://www.yeastgenome.org/reference/S000128634 Houseley et al 2008] PMID:19061643,
Pinskaya et al 2009 PMID:19407817, Geisler et al 2012 PMID:22226051 |
| II | YNCB0014W aka TBRT/XUT_2F-154 | New ncRNA | PMID:32081726 |
| III | RE/RE301 | New recombination enhancer | PMID:8861911 |
| V | YELWdelta27 | New Ty1 LTR | PMID:29320491 |
| V | HPA3/YEL066W | Moved translation start to Met19
old coordinates: 26667..27206 new coordinates: 26721..27206 |
PMID: 23775086 |
| VII | OTO1/YGR227C-A | New ORF | PMID: 25781884 |
| VII | ROK1/YGL171W | Added two new uORFs
uORF1: 182286..182407 uORF2: 182291..182329 |
PMID:20969870 |
| VIII | YHR052C-B | New ORF | PMID:29897761 |
| VIII | YHR054C-B | New ORF | PMID:29897761 |
| VIII | SUT169/YNCH0011W | New ncRNA | PMID:19169243, PMID:22226051, PMID:27292640, PMID:28977638 |
| X | YJR107C-A | New ORF | PMID:26554900, PMID:29897761 |
| X | YJR012C | Moved start to Met76
old coordinates: 459795..460418 Crick new coordinates: 459795..460193 Crick |
PMID:29632376 |
| XI | YKL104W-A | New ORF | PMID:29897761 |
| XII | YLR379W-A | ||
| XII | NTS1-2, NTS2-1, NTS2-2 | Change feature_type/SO_term from SO:0001637 rRNA_gene to SO:0000183 non_transcribed_region | |
| XIII | LDO45/YMR147W | Shift stop to be same as LDO16/YMR148W, add intron
old coordinates 559199..559870 new coordinates 559199..559780, 560156..560812 |
PMID:29187527 |
| XIII | YMR008C-A | ||
| XIII | YNCM0001W aka PHO84 lncRNA | New ncRNA | PMID:18022365 |
| XIV | LTO1/YNL260C | Move start to Met37
old coords: 156859..157455 Crick new coords: 156859..157347 Crick |
PMID: 26182403 |
| XVI | YNCP0002W aka GAL4 lncRNA | New ncRNA | PMID: 22226051 |
