Details of 2021 Reference Genome Annotation Update R64.3

The S. cerevisiae strain S288C reference genome annotation was updated in its first major update since 2014. The new genome annotation is release R64.3.1, dated 2021-04-22. Note that the underlying genome sequence itself was not altered in any way.

This annotation update included (details in table below):

• 5 new ncRNAs
• 1 new recombination enhancer
• 7 new ORFs
• 1 new LTR
• 2 new uORFs
• 2 ORFs with shifted translation starts
• 1 ORF with shifted translation stop plus new intron
• Changed feature_type (and SO_term) for non-transcribed spacers
• New systematic nomenclature system for entire annotated complement of ncRNAs

Chr	Feature	Description of change	Reference
II	YNCB0008W aka GAL10-ncRNA	New ncRNA	PMID:19061643, PMID:19407817, PMID:22226051
II	YNCB0014W aka TBRT/XUT_2F-154	New ncRNA	PMID:32081726
III	RE/RE301	New recombination enhancer	PMID:8861911
V	YELWdelta27	New Ty1 LTR	PMID:29320491
V	HPA3/YEL066W	Moved translation start to Met19 old coordinates: 26667..27206 new coordinates: 26721..27206	PMID: 23775086
VII	OTO1/YGR227C-A	New ORF	PMID: 25781884
VII	ROK1/YGL171W	Added two new uORFs uORF1: 182286..182407 uORF2: 182291..182329	PMID:20969870
VIII	YHR052C-B	New ORF	PMID:29897761
VIII	YHR054C-B	New ORF	PMID:29897761
VIII	SUT169/YNCH0011W	New ncRNA	PMID:19169243, PMID:22226051, PMID:27292640, PMID:28977638
X	YJR107C-A	New ORF	PMID:26554900, PMID:29897761
X	YJR012C	Moved start to Met76 old coordinates: 459795..460418 Crick new coordinates: 459795..460193 Crick	PMID:29632376
XI	YKL104W-A	New ORF	PMID:29897761
XII	YLR379W-A
XII	NTS1-2, NTS2-1, NTS2-2	Change feature_type/SO_term from SO:0001637 rRNA_gene to SO:0000183 non_transcribed_region
XIII	LDO45/YMR147W	Shift stop to be same as LDO16/YMR148W, add intron old coordinates 559199..559870 new coordinates 559199..559780, 560156..560812	PMID:29187527
XIII	YMR008C-A