Commonly used auxotrophic markers

This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in Brachmann et al. (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you have additions or modifications you would like to suggest.

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Allele	Deleted ORF?	Reverts?	Notes	Molecular Descriptiona	Reference
ade1-14	no	yes	red colonies	TGG-to-TGA nonsense change at codon 244; GGA-to-GAA missense change at codon 185	Nakayashiki et al. 2001
ade2-1	no	yes	ochre mutation	TTA-to-TTG silent change at codon 9, GAA-to-TAA ochre nonsense change at codon 64, AGA-to-GGA missense change at codon 101, GTT-to-GTC silent change at codon 124, ACG-to-ACA silent change at codon 539	Rodney Rothstein, Personal communication to SGD
ade2-101	no	yes	ochre mutation, red colonies	G to T transversion at nucleotide 190, changing codon 64 from a Glu to a Stop	Gai and Voytas, 2005
ade2-BglII	no	no	red colonies	frameshift (BglII site filled in at position 592)	Engebrecht and Roeder 1990
can1-100	no	yes	ochre mutation	AAA-to-TAA ochre nonsense change at codon 47	Rodney Rothstein, Personal communication to SGD
his3delta200	yes	no	Cold sensitive; high frequency of petite formation, especially during transformation. Note that this deletion damages the PET56 promoter. See Zhang et al. (2003) for a discussion of this issue.	1 kb deletion (-205 to 835)	Struhl 1985; Fasullo and Davis 1988
his3delta1	partial	no		187 bp HindIII-HindIII internal deletion (305 to 492)	Scherer and Davis 1979
his3-11,15	no	no	double mutant	G deletion at nucleotide 208, G deletion at nucleotide 319	Rodney Rothstein, Personal communication to SGD
leu2delta1	partial	no		EcoRI-ClaI internal deletion (163 to 649, 0.6 kb)	[www.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000044428 Sikorski and Hieter 1989]
leu2-3,112	no	no	double mutant	GTC-to-GTT silent change at codon 56, GTT-to-GCT missense change at codon 69, G insertion at nucleotide 249, G insertion at nucleotide 792, GTT-to-GTC silent change at codon 299, GAC-to-AAC missense change at codon 300	Hinnen et al. 1978; Gaber and Culbertson 1982; Meira LB et al., 1995; Rodney Rothstein, Personal communication to SGD
lys2-801	no	yes	amber mutation
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^aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.

^bAll trp- strains are cold sensitive (Singh and Manney 1974).