Commonly used auxotrophic markers

This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in Brachmann et al. (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you have additions or modifications you would like to suggest.

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Allele	Deleted ORF?	Reverts?	Molecular Descriptiona	Reference
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^aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.

^bAll trp- strains are cold sensitive (Singh and Manney 1974).