YJL156C
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| Systematic name | YJL156C |
| Gene name | SSY5 |
| Aliases | |
| Feature type | ORF, Verified |
| Coordinates | Chr X:128948..126849 |
| Primary SGDID | S000003692 |
Description of YJL156C: Serine protease of SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); contains an inhibitory domain that dissociates in response to extracellular amino acids, freeing a catalytic domain to activate transcription factor Stp1p[1][2][3][4]
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Community Commentary
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The Fungal Alignment Viewer at SGD indicates that SSY5/YJL156C in S. cerevisiae contains a frame-shift in the 4th-to-last codon (addition of an A), thereby truncating the protein relative to the other sensu stricto strains. In the other strains the ORF is a dozen amino acids longer. The protein and DNA sequences are highly conserved in this region, which contains a serine protease domain.
There are a couple of possibilities.
1) The reference genome S288C may contain this sequence and the gene is in fact shorter (687 amino acids).
2) The reference sequence for S288C is wrong. Only sequencing of the reference strain can tell.
This difference is cited in: Poulsen P, Wu B, Gaber RF, Kielland-Brandt MC. Constitutive signal transduction by mutant Ssy5p and Ptr3p components of the SPS amino acid sensor system in Saccharomyces cerevisiae. Eukaryot Cell. 2005 Jun;4(6):1116-24. PMID: 15947203
In this strain, the SSY5 ORF is 12 amino acids longer than the sequence in SGD.
Update: Option 2 has been confirmed. The additional A is erroneous and SSY5 is indeed 699 amino acids long. SGD plans to update SSY5 sequence in December, 2008.
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References
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- ↑ Andreasson C, et al. (2006) Regulation of transcription factor latency by receptor-activated proteolysis. Genes Dev 20(12):1563-8 SGD PMID 16778074
- ↑ Poulsen P, et al. (2006) Mapping of an internal protease cleavage site in the Ssy5p component of the amino acid sensor of Saccharomyces cerevisiae and functional characterization of the resulting pro- and protease domains by gain-of-function genetics. Eukaryot Cell 5(3):601-8 SGD PMID 16524914
- ↑ Forsberg H, et al. (2001) The role of the yeast plasma membrane SPS nutrient sensor in the metabolic response to extracellular amino acids. Mol Microbiol 42(1):215-28 SGD PMID 11679080
- ↑ Forsberg H and Ljungdahl PO (2001) Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids. Mol Cell Biol 21(3):814-26 SGD PMID 11154269
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References
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