Commonly used strains

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This page describes some of the most commonly used yeast lab strains. Much of the information is taken from F. Sherman (2002) Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include

  • Mortimer and Johnston (1986) Genetics 113:35-43 - thoroughly describes the genealogy of strain S288C
  • van Dijken et al. (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly used lab strains
  • Winzeler et al. (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identity between common lab strains

Strain backgrounds

S288C

Genotype: MATα SUC2 gal2 mal mel flo1 flo8-1 hap1

Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. S288C strains are gal2- and they do not use galactose anaerobically.

References: Mortimer and Johnston (1986) Genetics 113:35-43.

Sources:ATCC:204508

BY4743

Genotype: MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 MET15/met15Δ0 ura3Δ0/ura3Δ0

Notes:Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. See Brachmann et al. reference for details.

References: Brachmann et al. (1998) Yeast 14:115-32.

Sources: Biosystems:YSC1050

FY1679

Genotype: MATa/α ura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ1/LEU2 his3Δ200/HIS3 GAL2/GAL

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References:Winston et al. (1995) Yeast 11:53-55.

Sources: EUROSCARF:10000D

AB972

Genotype: MATα X2180-1B trp10 [rho 0]

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.

References: Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.

Sources: ATCC:204511

A364A

Genotype: MATa ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References: Hartwell (1967) J. Bacteriol. 93:1662-1670.

Sources: ATCC:208526

XJ24-24a

Genotype: MATa ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2

Notes: Derived from, but not isogenic to S288C

References: Strathern et al. (1979) Cell 18:309-319

DC5

Genotype: MATa leu2-3,112 his3-11,15 can1-11

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References: Broach et al. (1979) Gene 8:121-133

YNN216

Genotype: MATa/α ura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre

Notes: Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.

YPH499

Genotype: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes: Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note that trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker. gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

Sources: ATCC:204679

YPH500

Genotype: MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

Notes:MATα strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

Sources: ATCC:204680

YPH501

Genotype: MATa/MATα ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-Δ63/trp1-Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1

Notes:a/α diploid isogenic to YPH499 and YPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

Sources: ATCC:204681

Sigma 1278B

Notes:Used in pseudohyphal growth studies. Detailed notes about the sigma strains have been kindly provided by Cora Styles.

SK1

Genotype: MATa/α HO gal2 cupS can1R

Notes: Commonly used for studying sporulation or meiosis. Canavanine-resistant derivative

References: Kane SM and Roth J. (1974) Bacteriol. 118: 8-14

Sources: ATCC:204722

CEN.PK (aka CEN.PK2)

Genotype: MATa/α ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 Δ1/his3 Δ1 MAL2-8C/MAL2-8C SUC2/SUC2

References: van Dijken et al. (2000) Enzyme Microb Technol 26:706-714

Sources: EUROSCARF:30000D

W303

Genotype: MATa/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+]

Notes: W303 also contains a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele.

References: W303 constructed by Rodney Rothstein (see detailed notes from RR).
bud4 info: Voth, W.P. et al. (2005) Eukaryotic Cell, in press.
rad5-535 info: Fan et al. (1996) Genetics 142:749

Sources: Biosystems:YSC1058

W303-1A

Genotype: MATa {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}

Notes: W303-1A possesses a ybp1-1 mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.

References: W303 constructed by Rodney Rothstein (see detailed notes from RR).
ybp1-1 info: Veal et al. (2003) J. Biol. Chem. 278:30896-904.

Sources: Biosystems:YSC1058

W303-1B

Genotype: MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15}

References: W303 constructed by Rodney Rothstein (see detailed notes from RR).

Sources: Biosystems:YSC1058

X2180-1A

Genotype: MATa SUC2 mal mel gal2 CUP1

References: Robert Mortimer, personal comm.

Sources: ATCC:204504

D273-10B

Genotype: MATα mal

Notes: Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.

References: Sherman, F. (1963) Genetics 48:375-385.

Sources: ATCC:24657

FL100

Genotype: MATa

References: Lacroute, F. (1968) J. Bacteriol. 95:824-832.

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