UW-Stout/Formamide SP22

Wild yeast cell hi

Caution

Formamide can cause hi

Materials

• Neat Formamide, 33ul
• Sterile water, 567ul
• 0.2ml flat cap PCR tube, 13
• Well plate
• Wild yeast cells, 600ul

Formamide Concentrations

1. 0.5ul formamide + 49.5ul sterile water
2. 1.0ul formamide + 49.0ul sterile water
3. 1.5ul formamide + 48.5ul sterile water
4. 2.0ul formamide + 48.0ul sterile water
5. 2.5ul formamide + 47.5ul sterile water
6. 3.0ul formamide + 47.0ul sterile water
7. 3.5ul formamide + 46.5ul sterile water
8. 4.0ul formamide + 46.0ul sterile water
9. 4.5ul formamide + 45.5ul sterile water
10. 5.0ul formamide + 45.0ul sterile water
11. 5.5ul formamide + 44.5ul sterile water
12. 0.0ul formamide + 50.0ul sterile water

Procedure

1. Grab neat formamide and sterile water 2. Label 12 PCR tubes 1-12 and the 13th one place at least 33ul of neat formamide inside, can put excess in 3. Pipet appropriate amount of formamide and sterile water into the labeled PCR tubes according to number 4. In a sterile environment, pipet the liquid (50ul) from the PCR tube into the well cell as well as 50ul of the wild yeast cells 5. Incubate assess tray at 30 degrees Celsius for --- hours and record data hi