Positions in yeast labs

Postdoctoral position in synthetic biology. Montpellier, France. (posted january 20, 2017)

A post-doctoral position is available for 24 months at the “UMR-Sciences pour l’Œnologie de Montpellier” (UMR INRA 1083), in the Microbiology team, starting approximately in March 2017.

This work is part of a project financed by the “Agence Nationale de la Recherche” (ANR) (for details see ENZINVIVO project http://www.agence-nationale-recherche.fr/fileadmin/aap/2016/selection/aap-g-anr-DS10-selection-2016.pdf). Enzyme reactions have long been analyzed in vitro, using pure enzymes and diluted buffer conditions. Due to the large amount of data generated and collected with thousands of enzymes, enzymology has made tremendous progress on understanding the incredible power of biocatalysts. However, dilute, in vitro conditions are far from the surroundings of natural enzymatic reactions that take place inside cells. The cellular medium is more accurately described as a heterogeneous crowded gel, dense and filled with all sorts of macromolecules and cellular lipidic organelles which may result in some partitioning effects and changes in diffusion. Therefore, enzymatic parameters determined using classical enzymology setups may not perfectly represent the real, in vivo based, rate and equilibrium constants. Although some advances have been made toward the comprehension of viscosity and crowding effects, we are still far to derive rate and equilibrium parameters from in vivo enzymatic reactions. The project ENZINVIVO will address this issue focusing on two isoforms of phytoene synthase (carotenoid biosynthesis) as model enzymes. The enzymatic properties of these enzymes will be investigated in vitro, through a set of measurement in environments of increasing complexity and in vivo expressing the carotenoid biosynthesis pathway in S. cerevisiae and using synthetic biology tools and concepts to tune, at will, substrate and enzyme levels. We aim at deciphering: - how the intracellular medium influences enzymatic reactions. - how can we build an in vivo approach to measure enzymatic parameters. - how general models of enzymatic equations can be rewired to account for the complexity in vivo approaches. Our work will notably verify (or not) whether a Michaelis-Menten description of the kinetics remains valid in vivo. The candidate will be first responsible for the construction of engineered strains with fine-tuned or inducible expression of CtrE (geranylgeranyl pyrophosphate synthase, GGPP synthase) to modulate intracellular concentration of GGPP, substrate of the phytoene synthase in a range consistent with its KM. Then, he/she will be in charge of analyzing the consequences of the heterologous pathway on the yeast physiology through multi-levels characterization of the recombinant strains. Personal Qualifications • A PhD in the fields of molecular biology, genetics or related fields • Solid experience in metabolic engineering, omics approaches, and microbiology are highly desirable. • Skills in yeast metabolism and experience in multi-partners collaborations will be also strongly appreciated. • Ability to lead good oral and written communication skills, engaged and highly motivated.

Candidates please send a letter that describes your scientific interest and research experience, together with your CV, list of publications and the names of three references to Drs. Carole Camarasa (carole.camarasa@inra.fr) and Virginie Galeote (virginie.galeote@inra.fr).

Application deadline: 01/04/2017. Applications will be reviewed on an ongoing basis.

Multiple post-doctoral research positions in budding yeast cell cycle biology, Trento, Italy - (posted October 24th, 2016)

Multiple post-doctoral research positions are available in the laboratory of Prof. Peter De Wulf, which recently moved from the European Institute of Oncology (Milan, Italy) to the Centre for Integrative Biology (CIBIO), University of Trento (Trento, Italy (http://www.cibio.unitn.it)).

Available projects include the study of (i) how a minimally understood, conserved kinase regulates the timing of kinetochore assembly and re-organization during the S. cerevisiae cell cycle and (ii) how a novel and conserved ubiquitin-driven response pathway ensures kinetochore subunit homeostasis in S. cerevisiae.

Knowledge of molecular biology, biochemistry, fluorescence (live-cell) microscopy and/or genetics is required. Additional experience with yeast cell cycle biology (mitosis or meiosis) is an advantage. Please send your curriculum vitae to peter.dewulf@unitn.it. For more information about the lab, please see http://www.cibio.unitn.it/510/chromosome-segregation-biology

Postdoc Opening - Autophagy of lipid droplets in YEAST or zebrafish (posted 1 August 2016)

Postdoctoral project: Autophagy of lipid droplets in YEAST or zebrafish

Academic institution: University of California, San Diego

Academic division: Division of Biological Sciences

Academic unit: Section of Molecular Biology

Description: Applications are invited for a postdoctoral position in the group of Taras Nazarko studying mechanisms of lipophagy, the selective autophagy of lipid droplets (LDs). Lipophagy is accomplished by delivery of LDs from the cytosol to the lysosome (or vacuole in yeast). As in other autophagic pathways, the core autophagic machinery forms the autophagic isolation membrane that sequesters the LD from the cytosol. However, how this autophagic membrane recognizes the LD after lipophagy induction is unknown. Also, it is not clear how lipophagy is kept in check the rest of the time. Therefore, lipophagy selectivity and regulation are the key gaps in our understanding of this pathway. A postdoctoral scholar will develop a project in one of these areas. Mechanistic understanding in these areas is critical for the precise control of lipophagy in humans for the prevention and treatment of various lipid accumulation diseases, like atherosclerosis and obesity. Initial appointment is for 1 year with possible extension for up to 5 years of overall postdoctoral training. Salary is commensurate with experience (NIH NRSA scale).

Applicant requirements: The successful applicants will have a recent PhD in biochemistry, genetics, molecular or cell biology and a strong background in either YEAST or zebrafish genetics, protein biochemistry and fluorescence microscopy. Expertise with mammalian cells is a plus but not essential. Preference will be given to candidates with experience in AUTOPHAGY or LIPID DROPLETS.

How to apply: Please send your cover letter, CV and contact information of 3 references to Taras Nazarko, tnazarko@ucsd.edu

Postdoctoral Fellowship-Yeast Kinetochore Biology (posted 27 May 2016)

Postdoctoral Fellowship-Yeast Kinetochore Biology - The Francis Crick Institute, London.

Dr Thorpe’s laboratory focuses on kinetochore biology, mitosis and asymmetric cell division. Details of research projects currently being undertaken can be seen at: www.crick.ac.uk/peterthorpe. Research techniques used in the laboratory include high-throughput fluorescence imaging, genetics, genomics, and computational biology. The research aims to understand how kinetochores are assembled and maintained during cell division and to understand how kinetochore asymmetries affect chromosome segregation. In this project, some of the specific aims could include but are not limited to:
• Identifying regulators of kinetochore protein asymmetry.
• Characterise the role of protein modifications in directing kinetochore protein levels.
• Elucidate the role of cell cycle processes in maintaining the kinetochore.
• Examine the mechanistic links between chromatin and kinetochores.

PERSON SPECIFICATION

The post holder should embody and demonstrate our core Crick values: Bold, Imaginative, Open, Dynamic and Collegial, in addition to the following:

Essential
• PhD in cell biology/genetics or in the final stages of PhD submission.
• Good knowledge and experience in mathematical, statistical and data analysis.
• Technical expertise in microbiology and/or genetics.
• Track record of writing papers as evidenced by publications or submitted manuscripts in referred journals.
• Evidence of data presentation at scientific meetings.
• Experience of experimental design.
• Ability to work independently and also capable of interacting within a group.

Desirable
• Experience in genomics/high-throughput technologies.
• Experience in using computational biology to work with large data sets.
• Experience in synthetic biology and gene targeting.

Postdoctoral Training Fellows are expected to lead their own projects, contribute to other projects on a collaborative basis (both in the lab and with external collaborators) and guide PhD students in their research. The ability to work in a team is essential. Deadline for applications is 27th June 2016. More details and online application process at https://jobs.crick.ac.uk/pls/corehrrecruit/erq_jobspec_version_4.display_form?_company=1&p_internal_external=E&p_display_in_irish=N&p_applicant_no=&p_recruitment_id=002923&p_process_type=&p_form_profile_detail=&p_display_apply_ind=Y&p_refresh_search=Y

Postdoctoral Fellowship-Yeast Genetics meets protein therapeutics (posted 16 May 2016)

Postdoctoral Fellowship-Yeast Genetics meets protein therapeutics

The laboratories of Dr. Danny Chou and Jared Rutter in the Department of Biochemistry at the University of Utah are seeking a highly motivated postdoctoral researcher. This funded project focuses on using the awesome power of yeast genetics to discover and develop novel insulin variants with desirable therapeutic properties. This position will provide a unique opportunity to utilize experience with yeast genetics in developing new therapeutic agents for people with diabetes. A successful candidate must hold a Ph.D. in molecular biology, genetics or related fields. Preference will be given to those with experiences in yeast genetics. Candidates please send a letter that describes your scientific interest and research experience, together with your CV, list of publications and the names of three references to Drs. Danny Chou (dchou@biochem.utah.edu) and Jared Rutter (rutter@biochem.utah.edu).

Postdoctoral position in evolutionary systems biology. Québec, Canada. (posted Feb 23, 2016)

Postdoctoral position in evolutionary genomics and systems biology in the Landry Laboratory

A postdoctoral position is available in the Landry Laboratory at Université Laval in Québec City under the Canada Research Chair in Evolutionary Cell and Systems Biology. The PDF will work on a project at the interface of genomics, cell biology and evolution to investigate the mechanisms of evolution of gene and protein networks. The specific project will be developed with the selected candidate. The selected candidate will combine experimental evolution, high-throughput screening and bioinformatics, and the budding yeast Saccharomyces cerevisiae as experimental model system. The candidate is expected to have a PhD in biology or a related discipline, and a strong background in molecular and cell biology with at least basic skills in bioinformatics and statistics (R, Python or Perl). The candidate should have strong leadership skills, motivation and creativity and be able to work in a team of collaborators. The Landry lab is located at the Institut de Biologie Intégrative et des Systèmes (IBIS) of Université Laval and is part of the Quebec Network for Research on Protein Function, Engineering, and Applications (PROTEO). IBIS and PROTEO offer very stimulating training environment and cutting edge technologies in genomics and proteomics. The Landry lab is an international team of 15 students, PDFs and research associates from different backgrounds (microbiology, biology, bioinformatics, biochemistry) addressing questions in evolutionary cell and systems biology.

The application package (1 single PDF file) should include a motivation letter demonstrating the interest of the candidate for the field and his/her ability to perform this type of research, a short project proposal (half a page), reprints of the candidate’s most important contributions, a CV and the contact information of three people who can provide letters of reference. The file should be sent to landrylaboratory@gmail.com

Starting date could be as early as June 2016. The competition will remain open until a candidate is selected. For recent publications from the Landry lab, please visit: http://landrylab.ibis.ulaval.ca/

Postdoctoral position in Cell Biology and Genetics, Northern Kentucky University (posted May 7, 2015)

Laboratory of Erin Strome, Biological Sciences Department, Northern Kentucky University (Cincinnati Area)

A teaching-scholar faculty position is available to study mechanisms of haploinsufficiency induced genome instability. The position would be ideal for someone who will be pursuing a teaching and research balanced career and potentially be interested in a faculty job at an undergraduate institution. The postdoctoral fellow will have opportunities to develop their experimental and scientific credentials while also getting teaching experience and mentoring on teaching and lots of direct contact mentoring undergraduates in research lab projects.

Qualifications: Applicants should have a Ph.D. (ABD candidates will be considered) with a strong background in molecular biology/biochemistry/genetics and should be capable of conducting standard molecular biology tests including PCR and qPCR, Westerns, and siRNA experiments.

Please see https://jobs.nku.edu/postings/2817 for full job ad and application details.

Contact: Erin Strome, stromee1@nku.edu