YDR284C
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| Systematic name | YDR284C | |
| Gene name | DPP1 | |
| Aliases | ZRG1 | |
| Feature type | ORF, Verified | |
| Coordinates | Chr IV:1031415..1030546 | |
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Description of YDR284C: Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism[1][2][3][4][5][6]
Contents
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DNA and RNA Details
Other DNA and RNA Details
Other Topic: Transcription Regulation
DPP1 transcription is induced by the stationary phase of growth and by inositol supplementation. [7] [8]
Other
zinc regulation
DPP1-encoded diacylglycerol pyrophosphate phosphatase is induced by zinc depletion. This is dependent on Zap1p and the UASZRE in the DPP1 promoter [1] [9]
Protein Details
Protein Function/Process
Diacylglycerol pyrophosphate (DGPP)phosphatase, product of DPP1, purified to homogeneity. Pure enzyme utilizes DGPP and phosphatidic acid (PA) as substrates. DGPP is the preferred substrate with a 10-fold higher specificity constant. [2] [9]
DPP1-encoded diacylglycerol pyrophosphate (DGPP) phosphatase is induced by inositol supplementation and in stationary phase. DGPP phosphatase activity is inhibited by the phospholipid intermediate CDP-diacylglycerol. The DGPP molecule regulates phospholipid synthesis by activating phosphatidylserine synthase [7] [9]
The 3-domain lipid phosphatase motif in diacylglycerol pyrophosphate (DGPP) phosphatase is essential for catalytic activity using both DGPP and phosphatidic acid as substrates [4] [9]
DPP1 was identified based on the protein sequence of diacylglycerol pyrophosphate (DGPP) phosphatase. Accounts for nearly all of the DGPP phosphatase activity in the cell. Remaining DGPP phosphatase activity is due to the LPP1-encoded enzyme. DPP1 gene product also exhibits phosphatidic acid phosphatase activity, but with a 10-fold lower specificity. [5] [9]
Protein Localization
The DPP1-encoded diacylglycerol pyrophosphate (DGPP) phosphatase is localized to the vacuolar membrane. [1] [9]
References
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- ↑ 1.0 1.1 1.2 Han GS, et al. (2001) Regulation of the Saccharomyces cerevisiae DPP1-encoded diacylglycerol pyrophosphate phosphatase by zinc. J Biol Chem 276(13):10126-33 SGD PMID 11139591
- ↑ 2.0 2.1 Wu WI, et al. (1996) Purification and characterization of diacylglycerol pyrophosphate phosphatase from Saccharomyces cerevisiae. J Biol Chem 271(4):1868-76 SGD PMID 8567632
- ↑ Furneisen JM and Carman GM (2000) Enzymological properties of the LPP1-encoded lipid phosphatase from Saccharomyces cerevisiae. Biochim Biophys Acta 1484(1):71-82 SGD PMID 10685032
- ↑ 4.0 4.1 Toke DA, et al. (1999) Mutagenesis of the phosphatase sequence motif in diacylglycerol pyrophosphate phosphatase from Saccharomyces cerevisiae. Biochemistry 38(44):14606-13 SGD PMID 10545184
- ↑ 5.0 5.1 Toke DA, et al. (1998) Isolation and characterization of the Saccharomyces cerevisiae DPP1 gene encoding diacylglycerol pyrophosphate phosphatase. J Biol Chem 273(6):3278-84 SGD PMID 9452443
- ↑ Yuan DS (2000) Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging. Genetics 156(1):45-58 SGD PMID 10978274
- ↑ 7.0 7.1 Oshiro J, et al. (2000) Regulation of the DPP1-encoded diacylglycerol pyrophosphate (DGPP) phosphatase by inositol and growth phase. Inhibition of DGPP phosphatase activity by CDP-diacylglyceron and activation of phosphatidylserine synthase activity by DGPP. J Biol Chem 275(52):40887-96 SGD PMID 11016943
- ↑ submitted by June Oshiro on 2003-03-24
- ↑ 9.0 9.1 9.2 9.3 9.4 9.5 submitted by George M. Carman on 2003-04-04
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