YHR048W
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Systematic name | YHR048W |
Gene name | YHK8 |
Aliases | |
Feature type | ORF, Uncharacterized |
Coordinates | Chr VIII:204607..206151 |
Primary SGDID | S000001090 |
Description of YHR048W: Presumed antiporter of the DHA1 family of multidrug resistance transporters; contains 12 predicted transmembrane spans; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles[1][2]
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Contents
Community Commentary
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DNA and RNA Details
Other DNA and RNA Details
Other Topic: expression
Specifically lower expression in carbon limited chemostat cultures versus carbon excess. [3] [4]
Growth in YPD
In a BY4735 background, knocking out YHR048 seems to have little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YHR048W knock-out strain's doubling time was 432 minutes. (These doubling times are the means of three experiments.)
Caffeine and Yeast Cells
This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 801.97 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.
The protocol can be found at
Caffeine
This bar graph shows that compared to a BY4735 background, Knocking out yHR048c makes yeast less sensitive to UV light exposure
The protocol can be found at
UW_Stout/UV_Light_FA19UV Light Exposure
Cycloheximide effect on YHR048W
As you can see from above test 4 thrived, it did very good, but test 1-3 did not do as well and were all lower than the growth curve. Growth models 1-3 are YHR048W without any stress induced.
Ethanol Tolerance in YHR048W
This graph shows the growth between YHR048W treated and untreated with ethanol. The rate of growth of the yeast treated with ethanol is (answer). The rate of growth of the yeast not treated with ethanol is (answer). This shows that the strands of YHR048W treated with ethanol have an increased growth rate than the ones that weren't.
Prednisone Effects on YHR048W
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References
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- ↑ Barker KS, et al. (2003) Identification of genes differentially expressed in association with reduced azole susceptibility in Saccharomyces cerevisiae. J Antimicrob Chemother 51(5):1131-40 SGD PMID 12697649
- ↑ Gbelska Y, et al. (2006) Evolution of gene families: the multidrug resistance transporter genes in five related yeast species. FEMS Yeast Res 6(3):345-55 SGD PMID 16630275
- ↑ Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
- ↑ submitted by Viktor Boer on 2003-07-25
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