Difference between revisions of "Commonly used strains"

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* [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000080159 Winzeler et al.] (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identity between common lab strains
 
* [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000080159 Winzeler et al.] (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identity between common lab strains
  
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== Strains described on this page ==
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= Strain backgrounds =
  
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{|
 
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* [http://wiki.yeastgenome.org/index.php/straintable#A364A A364A]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#AB972 AB972]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#BY4743 BY4743]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#CEN.PK2 CEN.PK/CEN.PK2]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#D273-10B D273-10B]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#DC5 DC5]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#FL100 FL100]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#FY1679 FY1679]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#S288C S288C]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#Sigma_1278B Sigma 1278B]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#SK1 SK1]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#W303 W303]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#X2180-1A X2180-1A]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#XJ24-24a XJ24-24a]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#YNN216 YNN216]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#YPH499 YPH499]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#YPH500 YPH500]
 
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* [http://wiki.yeastgenome.org/index.php/straintable#YPH501 YPH501]
 
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|}
 
  
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=S288C=
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'''Genotype:''' ''MAT''? ''SUC2 gal2 mal mel flo1 flo8-1 hap1''
  
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'''Notes:''' Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of [http://db.yeastgenome.org/cgi-bin/locus.pl?dbid=S000004246 ''HAP1''], it is not a good strain for mitochondrial studies. S288C strains are ''gal''2- and they do not use galactose anaerobically.
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'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000050744 Mortimer and Johnston] (1986) Genetics 113:35-43.
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'''Sources:'''[http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=204508 ATCC: 204508]
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=FY1679=
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'''Genotype:''' ''MAT''a/? ''ura3-52/ura3-52 trp1''?''63/TRP1 leu2''?'' 1/LEU2 his3''?''200/HIS3 GAL2/GAL''
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'''Notes:'''  Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.
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'''References:'''[http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000047446 Winston et al.] (1995) Yeast 11:53-55.
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'''Sources:''' [http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/fy1679.html EUROSCARF: 10000D]
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=BY4743=
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'''Genotype:''' ''MAT''a/? ''his3''?''1/his3''?''1 leu2''?''0/leu2''?''0 lys2''?''0/LYS2 MET15/met15''?''0 ura3''?''0/ura3''?''0''
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'''Notes:'''Strain used in the [http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www-sequence.stanford.edu/group/yeast_deletion_project/project_desc.html systematic deletion project], generated from a cross between BY4741 and BY4742, which are derived from S288C. See Brachmann et al. reference for details.
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'''References:''' [http://db.yeastgenome.org/cgi-bin/reference/reference.pl?dbid=S000041186 Brachmann et al.] (1998) Yeast 14:115-32.
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'''Sources:''' [http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.openbiosystems.com/ Open Biosystems: YSC1050]
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=W303=
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'''Genotype:''' ''MAT''a/''MAT''? {''leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15''} [''phi<sup>+</sup>'']
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'''Notes:''' W303 also contains a ''bud4'' mutation that causes
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haploids to bud with a mixture of axial and bipolar budding
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patterns. In addition, the original W303 strain contains the
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''rad5-535'' allele.
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'''References:''' W303 constructed by Rodney Rothstein (''see [http://wiki.yeastgenome.org/index.php/CommunityW303.html detailed notes] from RR'').<br />''bud4'' info: Voth, W.P. et al. (2005) Eukaryotic Cell, in press. <br />''rad5-535'' info: Fan et al. (1996) Genetics 142:749
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'''Sources:''' [http://db.yeastgenome.org/cgi-bin/redirect.pl?source=straintable&url=http://www.openbiosystems.com/Genomics/Model Organism Resources/Yeast Resources/Yeast Knockout Strains/ Open Biosystems: YSC1058]
  
 
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Revision as of 15:15, 20 July 2007

This table describes some of the most commonly used yeast lab strains. Much of the information is taken from F. Sherman (2002) Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include

  • Mortimer and Johnston (1986) Genetics 113:35-43 - thoroughly describes the genealogy of strain S288C
  • van Dijken et al. (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly used lab strains
  • Winzeler et al. (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic identity between common lab strains

Strain backgrounds

S288C

Genotype: MAT? SUC2 gal2 mal mel flo1 flo8-1 hap1

Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. S288C strains are gal2- and they do not use galactose anaerobically.

References: Mortimer and Johnston (1986) Genetics 113:35-43.

Sources:ATCC: 204508

FY1679

Genotype: MATa/? ura3-52/ura3-52 trp1?63/TRP1 leu2? 1/LEU2 his3?200/HIS3 GAL2/GAL

Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

References:Winston et al. (1995) Yeast 11:53-55.

Sources: EUROSCARF: 10000D

BY4743

Genotype: MATa/? his3?1/his3?1 leu2?0/leu2?0 lys2?0/LYS2 MET15/met15?0 ura3?0/ura3?0

Notes:Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. See Brachmann et al. reference for details.

References: Brachmann et al. (1998) Yeast 14:115-32.

Sources: Open Biosystems: YSC1050

W303

Genotype: MATa/MAT? {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+]

Notes: W303 also contains a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele.

References: W303 constructed by Rodney Rothstein (see detailed notes from RR).
bud4 info: Voth, W.P. et al. (2005) Eukaryotic Cell, in press.
rad5-535 info: Fan et al. (1996) Genetics 142:749

Sources: Organism Resources/Yeast Resources/Yeast Knockout Strains/ Open Biosystems: YSC1058

Strain Genotype Notes References ATCC/Open Biosystems/EUROSCARF
S288C
MATα SUC2 gal2 mal mel flo1 flo8-1 hap1

Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mitochondrial studies. S288C strains are gal2- and they do not use galactose anaerobically.

Mortimer and Johnston (1986) Genetics 113:35-43.

ATCC: 204508

FY1679
MATa/α ura3-52/ura3-52 trp1Δ63/TRP1 leu2Δ 1/LEU2 his3Δ200/HIS3 GAL2/GAL Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

Winston et al. (1995) Yeast 11:53-55.

EUROSCARF: 10000D

BY4743
MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 lys2Δ0/LYS2 MET15/met15Δ0 ura3Δ0/ura3Δ0

Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. See Brachmann et al. reference for details.

Brachmann et al. (1998) Yeast 14:115-32.

Open Biosystems: YSC1050

W303
MATa/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+] W303 also contains a bud4 mutation that causes haploids to bud with a mixture of axial and bipolar budding patterns. In addition, the original W303 strain contains the rad5-535 allele. W303 constructed by Rodney Rothstein (see detailed notes from RR).
bud4 info: Voth, W.P. et al. (2005) Eukaryotic Cell, in press.
rad5-535 info: Fan et al. (1996) Genetics 142:749

Organism Resources/Yeast Resources/Yeast Knockout Strains/ Open Biosystems: YSC1058

W303-1A
MATa {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} W303-1A possesses a ybp1-1 mutation (I7L, F328V, K343E, N571D) which abolishes Ybp1p function, increasing sensitivity to oxidative stress.

W303 constructed by Rodney Rothstein (see detailed notes from RR).
ybp1-1 info: Veal et al. (2003) J. Biol. Chem. 278:30896-904.

Organism Resources/Yeast Resources/Yeast Knockout Strains/ Open Biosystems: YSC1058

W303-1B
MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} W303 constructed by Rodney Rothstein (see detailed notes from RR).

Organism Resources/Yeast Resources/Yeast Knockout Strains/ Open Biosystems: YSC1058

X2180-1A
MATa SUC2 mal mel gal2 CUP1 Robert Mortimer, personal comm.

ATCC: 204504

AB972
MATα X2180-1B trp10 [rho 0] Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.

Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.

ATCC: 204511

A364A
MATa ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

Hartwell (1967) J. Bacteriol. 93:1662-1670.

ATCC: 208526

Sigma 1278B

Used in pseudohyphal growth studies. Detailed notes about the sigma strains have been kindly provided by Cora Styles.

SK1 (canavanine-resistant derivative)
MATa/α HO gal2 cupS can1R Commonly used for studying sporulation or meiosis

Kane SM and Roth J. (1974) Bacteriol. 118: 8-14

ATCC: 204722

CEN.PK (aka CEN.PK2)
MATa/α ura3-52/ura3-52 trp1-289/trp1-289 leu2-3_112/leu2-3_112 his3 Δ1/his3 Δ1 MAL2-8C/MAL2-8C SUC2/SUC2

van Dijken et al. (2000) Enzyme Microb Technol 26:706-714

EUROSCARF: 30000D

XJ24-24a
MATa ho HMa HMα ade6 arg4-17 trp1-1 tyr7-1 MAL2 Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

Strathern et al. (1979) Cell 18:309-319

DC5
MATa leu2-3,112 his3-11,15 can1-11 Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.

Broach et al. (1979) Gene 8:121-133

YNN216
MATa/α ura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochre Congenic to S288C (see Sikorski and Hieter). Used to derive YSS and CY strains (see Sobel and Wolin).

Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.

D273-10B
MATα mal Normal cytochrome content and respiration; low frequency of rho-. This strain and its auxotrophic derivatives were used in numerious laboratories for mitochondrial and related studies and for mutant screens. Good respirer that's relatively resistant to glucose repression.

Sherman, F. (1963) Genetics 48:375-385.

ATCC: 24657

FL100
MATa

Lacroute, F. (1968) J. Bacteriol. 95:824-832.

YPH499
MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1 Contains nonrevertible (deletion) auxotrophic mutations that can be used for selection of vectors. Note that trp1-Δ63, unlike trp1-Δ1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker. gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

ATCC: 204679

YPH500
MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1

MATα strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

ATCC:204680

YPH501
MATa/MATα ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-Δ63/trp1-Δ63 his3-Δ200/his3-Δ200 leu2-Δ1/leu2-Δ1

a/α diploid isogenic to YPH499 and YPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.

Sikorski RS and Hieter P (1989) Genetics 122:19-27.
Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.
Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.

ATCC:204681

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