Difference between revisions of "UW-Stout/Saline SP23"
| Line 2: | Line 2: | ||
'''Saltwater Torture''' | '''Saltwater Torture''' | ||
| − | Introduction: | + | '''Introduction''': |
This experiment is testing how 2 M saltwater has a differing effect on the growth of yeast cells with different genes edited in. | This experiment is testing how 2 M saltwater has a differing effect on the growth of yeast cells with different genes edited in. | ||
| − | Materials | + | |
| + | '''Materials''' | ||
*H2O | *H2O | ||
*NaCl (table salt) | *NaCl (table salt) | ||
| Line 11: | Line 12: | ||
*Yeast cells (wild, 1, 2, & 3) | *Yeast cells (wild, 1, 2, & 3) | ||
*Microwell plate | *Microwell plate | ||
| − | Procedure: | + | |
| + | '''Procedure''': | ||
#Obtain a sample of 2 M saltwater. | #Obtain a sample of 2 M saltwater. | ||
#Obtain the yeast cells | #Obtain the yeast cells | ||
Revision as of 15:34, 6 May 2023
Saltwater Torture
Introduction: This experiment is testing how 2 M saltwater has a differing effect on the growth of yeast cells with different genes edited in.
Materials
- H2O
- NaCl (table salt)
- 2 M saltwater
- micropipettes
- Yeast cells (wild, 1, 2, & 3)
- Microwell plate
Procedure:
- Obtain a sample of 2 M saltwater.
- Obtain the yeast cells
- Pick a row in the microwell plate and micropipette 50 ul of 2 M saltwater into the first four wells of the row.
- Micropipette 50 ul of each of the yeast cells into the wells with the saltwater.
- Record where you put each sample.
- Put into plate reader for 18-24 hours.
- Obtain results from plate reader and calculate the doubling rate.
