Positions in yeast labs

Post-doc Opening - quantitative genetics of drug resistance in Saccharomyces cerevisiae

Project Description A post-doc position is open in the laboratory of John McCusker (https://mgm.duke.edu/faculty-and-research/primary-faculty/john-h-mccusker-phd/) in the Department of Molecular Genetics & Microbiology at Duke University Medical Center on a newly funded R01 to work on the quantitative genetics of drug resistance in Saccharomyces cerevisiae. The post-doc position requires demonstrated productivity (i.e. publications) as well as expertise in genetics (preferably microbial, ideally with S. cerevisiae) and in molecular biology. Please provide curriculum vitae, as well as the names and contact information for three references.

Applications must be made via Academic Jobs Online (https://academicjobsonline.org/ajo/jobs/9718).

Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual’s age, color, disability, genetic information, gender, gender identity, gender expression, national origin, race, religion, sexual orientation, or veteran status.

Postdoc Opening - Autophagy of lipid droplets in YEAST (posted 29 August 2017)

Postdoctoral project: Autophagy of lipid droplets in YEAST

Academic institution: University of California, San Diego

Academic division: Division of Biological Sciences

Academic unit: Section of Molecular Biology

Description: Applications are invited for a postdoctoral position in the group of Taras Nazarko studying mechanisms of lipophagy, the selective autophagy of lipid droplets (LDs). Lipophagy is accomplished by delivery of LDs from the cytosol to the lysosome (or vacuole in yeast). As in other autophagic pathways, the core autophagic machinery forms the autophagic isolation membrane that sequesters the LD from the cytosol. However, how this autophagic membrane recognizes the LD after lipophagy induction is unknown. Also, it is not clear how lipophagy is kept in check the rest of the time. Therefore, lipophagy selectivity and regulation are the key gaps in our understanding of this pathway. A postdoctoral scholar will develop a project in one of these areas. Mechanistic understanding in these areas is critical for the precise control of lipophagy in humans for the prevention and treatment of various lipid accumulation diseases, like atherosclerosis and obesity. Initial appointment is for 1 year with possible extension for up to 5 years of overall postdoctoral training. Salary is commensurate with experience (http://postdoc.ucsd.edu/appointment-guidelines/).

Applicant requirements: The successful applicants will have a recent PhD in biochemistry, genetics, molecular or cell biology and a strong background in YEAST genetics, protein biochemistry and fluorescence microscopy. Expertise with mammalian cells or zebrafish model is a plus but not essential. Preference will be given to candidates with experience in autophagy pathway or with LDs.

How to apply: Please send your cover letter, CV and contact information of 3 references to Taras Nazarko, tnazarko@ucsd.edu

Postdoc Position in Non-coding Transcription in Non-coding Transcription at University of Copenhagen, Denmark (posted April 25, 2017)

Copenhagen Plant Science Centre (CPSC) at the University of Copenhagen is seeking a postdoc commencing 1st September 2017 or as soon as possible thereafter. CPSC is a new initiative to promote excellent training opportunities in a modern research environment in the heart of Copenhagen. The position is for 2 years with the possibility of extension. A Hallas-Møller Investigator Award to Sebastian Marquardt funds the position. http://novonordiskfonden.dk/en/content/hallas-m%C3%B8ller-scholarship-denmark.

Project description The Marquardt lab is interested in the functional significance of abundant yet mysterious non-coding sequences present in genomes. http://cpsc.ku.dk/meet-the-scientists-page/sebastian-marquardts-group/ . We are looking for a postdoc to work on two lab focus areas within this field:

- Divergent lncRNA Transcription (1)
- Functional Consequences of Non-Coding Transcription (2, 3)

The position builds on unpublished data of the lab. We have identified novel factors controlling transcription of non-coding sequences in budding yeast. You will determine the genome-wide effect of these factors by suitable cutting-edge transcriptomics techniques. You will help to elucidate the molecular mechanisms that are required for divergent non-coding transcription from gene promoters. We use the knowledge of molecular mechanisms to study the functional roles of non-coding transcription in yeast and plants.

We are seeking an enthusiastic candidate familiar with high-throughput approaches, ideally with topically relevant research background. Please apply via the Copenhagen University job portal, where you can also find further information and application requirements:

http://jobportal.ku.dk/alle-opslag/?show=904563

Deadline: 15th June 2017.

(1) Marquardt et al. Cell. 2014 (2) Marquardt et al. Mol Cell. 2014 (3) *Liu, *Marquardt, et al. Science. 2010 * Joint first authors

Ph.D. Fellow in Non-coding Transcription at University of Copenhagen, Denmark (posted April 25, 2017)

Project description Copenhagen Plant Science Centre (CPSC) at the University of Copenhagen is offering a 3-year Ph.D.-fellowship commencing 1st September 2017 or as soon as possible thereafter. CPSC is a new initiative to promote excellent training opportunities in a modern research environment in the heart of Copenhagen. The position is funded by a Hallas-Møller Investigator Award to Sebastian Marquardt. http://novonordiskfonden.dk/en/content/hallas-m%C3%B8ller-scholarship-denmark

The Marquardt lab is interested in the functional significance of abundant yet mysterious non-coding sequences present in genomes. http://cpsc.ku.dk/meet-the-scientists-page/sebastian-marquardts-group/ . Our preliminary data support a Ph.D. position in any of these three focus areas within non-coding transcription research:

- Divergent lncRNA Transcription (1)
- Functional Consequences of Non-Coding Transcription (2, 3)
- Transcription Kinetics in Environmental Interactions (4)

Please specify in your Cover Letter what attracts you for a Ph. D. in any of these focus areas. Our lab employs cutting edge budding yeast technology to identify the molecular mechanisms controlling transcription of non-coding sequences. The knowledge of non-coding transcription mechanisms helps us to study the functional roles of non-coding transcription. For example, we disrupt non-coding transcription in Arabidopsis to identify roles of non-coding transcription in plant environmental responses. http://cpsc.ku.dk/meet-the-scientists-page/sebastian-marquardts-group/

A successful candidate will be enthusiastic about the general research area, ideally with relevant research background. Please apply via the Copenhagen University job portal, where you can also find further information and requirements: http://jobportal.ku.dk/alle-opslag/?show=904540

Deadline: 15th June 2017.

(1) Marquardt et al. Cell. 2014 (2) Marquardt et al. Mol Cell. 2014 (3) *Liu, *Marquardt, et al. Science. 2010 * Joint first authors (4) Hazelbaker, Marquardt, et al. Mol Cell. 2013

Postdoctoral position in synthetic biology. Montpellier, France. (posted january 20, 2017)

A post-doctoral position is available for 24 months at the “UMR-Sciences pour l’Œnologie de Montpellier” (UMR INRA 1083), in the Microbiology team, starting approximately in March 2017.

This work is part of a project financed by the “Agence Nationale de la Recherche” (ANR) (for details see ENZINVIVO project http://www.agence-nationale-recherche.fr/fileadmin/aap/2016/selection/aap-g-anr-DS10-selection-2016.pdf). Enzyme reactions have long been analyzed in vitro, using pure enzymes and diluted buffer conditions. Due to the large amount of data generated and collected with thousands of enzymes, enzymology has made tremendous progress on understanding the incredible power of biocatalysts. However, dilute, in vitro conditions are far from the surroundings of natural enzymatic reactions that take place inside cells. The cellular medium is more accurately described as a heterogeneous crowded gel, dense and filled with all sorts of macromolecules and cellular lipidic organelles which may result in some partitioning effects and changes in diffusion. Therefore, enzymatic parameters determined using classical enzymology setups may not perfectly represent the real, in vivo based, rate and equilibrium constants. Although some advances have been made toward the comprehension of viscosity and crowding effects, we are still far to derive rate and equilibrium parameters from in vivo enzymatic reactions. The project ENZINVIVO will address this issue focusing on two isoforms of phytoene synthase (carotenoid biosynthesis) as model enzymes. The enzymatic properties of these enzymes will be investigated in vitro, through a set of measurement in environments of increasing complexity and in vivo expressing the carotenoid biosynthesis pathway in S. cerevisiae and using synthetic biology tools and concepts to tune, at will, substrate and enzyme levels. We aim at deciphering: - how the intracellular medium influences enzymatic reactions. - how can we build an in vivo approach to measure enzymatic parameters. - how general models of enzymatic equations can be rewired to account for the complexity in vivo approaches. Our work will notably verify (or not) whether a Michaelis-Menten description of the kinetics remains valid in vivo. The candidate will be first responsible for the construction of engineered strains with fine-tuned or inducible expression of CtrE (geranylgeranyl pyrophosphate synthase, GGPP synthase) to modulate intracellular concentration of GGPP, substrate of the phytoene synthase in a range consistent with its KM. Then, he/she will be in charge of analyzing the consequences of the heterologous pathway on the yeast physiology through multi-levels characterization of the recombinant strains. Personal Qualifications • A PhD in the fields of molecular biology, genetics or related fields • Solid experience in metabolic engineering, omics approaches, and microbiology are highly desirable. • Skills in yeast metabolism and experience in multi-partners collaborations will be also strongly appreciated. • Ability to lead good oral and written communication skills, engaged and highly motivated.

Candidates please send a letter that describes your scientific interest and research experience, together with your CV, list of publications and the names of three references to Drs. Carole Camarasa (carole.camarasa@inra.fr) and Virginie Galeote (virginie.galeote@inra.fr).

Application deadline: 01/04/2017. Applications will be reviewed on an ongoing basis.