Reagents

• Cora Styles Lab Supplies - Simple, high-quality yeast dissection and replica plating tools, including yeast dissection needle kits, velveteens, and replica plating blocks.

• Antibodies made to S. cerevisiae proteins

• Custom Oligos and Primers - Several companies provide custom oligo synthesis, including but not limited to, those listed here:
  • GeneWorks
  • Integrated DNA Technologies
  • Invitrogen
  • Find other companies by searching Google

• Primer Set Sequences Links to download the sequences of primer sets from ResGen/Invitrogen and from the Yeast Knockout (YKO) Consortium

• MORF collection is a moveable ORF library which enables protein expression and purification for more than 4900 S. cerevisiae genes, as described in Gelperin DM, et al. (2005).

• pAG Vectors is a collection of 288 yeast Gateway expression vectors. These "Advanced Gateway" vectors are modifications of the pRS series plasmids and allow for inducible or constitutive expression, as well as fusion to a number of protein tags and fluorescent proteins. We have made all of these vectors available through the non-profit plasmid repository Addgene. Clones were created by the Lindquist Lab at the Whitehead Institute. "A suite of Gateway vectors for high-throughput genetic analysis in Saccharomyces cerevisiae". Simon Alberti, Aaron D. Gitler, and Susan Lindquist. 2007. Yeast. In Press.

• The PlasmID repository distributes over 5,200 fully sequence-verified S. cerevisiae genes in Gateway entry vectors and expression vectors. The PlasmID repository is part of the DF/HCC DNA Resource Core at the Harvard Institute of Proteomics at Harvard Medical School.

• The DNASU Plasmid Repository stores and distributes nearly 100,000 plasmids including over 35,000 human and mouse plasmids, full genome collections of numerous organisms including S. cerevisiae, the protein expression plasmids from the Protein Structure Initiative Material Repository (PSI-MR), and both small and large collections from individual researchers. DNASU accepts plasmid contributions from individual researchers and will distribute them to the community.

• Tritech Research maintains a list of links to S. cerevisiae information and protocols, and sells reagents and equipment for S. cerevisiae research.

• VectorDB, collected sequences of cloning vectors for use in a variety of organisms.

• Addgene: A Better Way to Share Plasmids. Addgene is a non-profit plasmid repository dedicated to helping academic scientists around the world share high-quality plasmids.

• Microarray methods for profiling the Yeast Knockout (YKO) Collection