Difference between revisions of "YHR096C"

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Specifically higher expression in carbon limited chemostat cultures versus carbon excess.
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<ref>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur.
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J Biol Chem 278(5):3265-74</ref>
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Revision as of 12:02, 21 February 2007

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Systematic name YHR096C
Gene name HXT5
Aliases
Feature type ORF, Verified
Coordinates Chr VIII:296450..294672


Description of YHR096C: Hexose transporter with moderate affinity for glucose, induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs[1][2][3]




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Community Commentary

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: CEN.PK2-1C
Genotype: EBY4000 (hxt)
Together with: HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT12, HXT8, HXT9, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11
Phenotype(s): Viable , Conditionally inviable when on glucose medium

The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. [4] [5]


DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically higher expression in carbon limited chemostat cultures versus carbon excess. [6] [7]


Protein Details

Protein Function/Process

The Hxt5 glucose transporter is one of four Hxt transporters (Hxt4p, Hxt5p, Hxt7p, Gal2p) able to take up xylose [8] [5]




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References

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  1. Greatrix BW and van Vuuren HJ (2006) Expression of the HXT13, HXT15 and HXT17 genes in Saccharomyces cerevisiae and stabilization of the HXT1 gene transcript by sugar-induced osmotic stress. Curr Genet 49(4):205-17 SGD PMID 16397765
  2. Verwaal R, et al. (2002) HXT5 expression is determined by growth rates in Saccharomyces cerevisiae. Yeast 19(12):1029-38 SGD PMID 12210898
  3. Diderich JA, et al. (2001) Functional analysis of the hexose transporter homologue HXT5 in Saccharomyces cerevisiae. Yeast 18(16):1515-24 SGD PMID 11748728
  4. Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 SGD PMID 10618490
  5. 5.0 5.1 submitted by Eckhard Boles on 2003-03-17
  6. Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  7. submitted by Viktor Boer on 2003-07-25
  8. Hamacher T, et al. (2002) Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization. Microbiology 148(Pt 9):2783-8 SGD PMID 12213924

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