Commonly used auxotrophic markers

This table describes some commonly used auxotrophic markers (along with some novel useful markers); it is based on a table in Brachmann et al. (1998) "Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications." Yeast 14:115-132. Please send e-mail to the curators at SGD at sgd-helpdesk@lists.stanford.edu if you have additions or modifications you would like to suggest.

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Allele	Deleted ORF?	Reverts?	Notes	Molecular Descriptiona	Reference
ade1-14	no	yes	red colonies	TGG-to-TGA nonsense change at codon 244; GGA-to-GAA missense change at codon 185	Nakayashiki et al. 2001
ade2-1	no	yes	ochre mutation	TTA-to-TTG silent change at codon 9, GAA-to-TAA ochre nonsense change at codon 64, AGA-to-GGA missense change at codon 101, GTT-to-GTC silent change at codon 124, ACG-to-ACA silent change at codon 539	Rodney Rothstein, Personal communication to SGD
ade2-101	no	yes	ochre mutation, red colonies	G to T transversion at nucleotide 190, changing codon 64 from a Glu to a Stop	Gai and Voytas, 2005
ade2-BglII	no	no	red colonies	frameshift (BglII site filled in at position 592)	Engebrecht and Roeder 1990
can1-100	no	yes	ochre mutation	AAA-to-TAA ochre nonsense change at codon 47	Rodney Rothstein, Personal communication to SGD
his3delta200	yes	no	Cold sensitive; high frequency of petite formation, especially during transformation. Note that this deletion damages the PET56 promoter. See Zhang et al. (2003) for a discussion of this issue.	1 kb deletion (-205 to 835)	Struhl 1985; Fasullo and Davis 1988
his3delta1	partial	no		187 bp HindIII-HindIII internal deletion (305 to 492)	Scherer and Davis 1979
his3-11,15	no	no	double mutant	G deletion at nucleotide 208, G deletion at nucleotide 319	Rodney Rothstein, Personal communication to SGD
leu2delta1	partial	no		EcoRI-ClaI internal deletion (163 to 649, 0.6 kb)	Sikorski and Hieter 1989
leu2-3,112	no	no	double mutant	GTC-to-GTT silent change at codon 56, GTT-to-GCT missense change at codon 69, G insertion at nucleotide 249, G insertion at nucleotide 792, GTT-to-GTC silent change at codon 299, GAC-to-AAC missense change at codon 300	Hinnen et al. 1978; Gaber and Culbertson 1982; Meira LB et al., 1995; Rodney Rothstein, Personal communication to SGD
lys2-801	no	yes	amber mutation
lys2delta202	partial	no		XhoI-HpaI internal deletion (1813 to 2864, 1.0 kb)	Winston et al. 1995
trp1delta1	yes	no	cold sensitiveb, weak galactose inducer (deletes GAL3 UAS), removes ARS1, also called trp1-901	1.45 kb deletion, EcoRI-EcoRI (-102 to 1352)	Sikorski and Hieter 1989
trp1delta63	partial	no	cold sensitiveb	0.6 kb deletion, EcoRI-HindIII (-102 to 513)	Sikorski and Hieter 1989
trp1-1	no	yes	amber mutation	GAG-to-TAG amber nonsense change at codon 83	McDonald et al. 1997
trp1-1	no	yes	cold sensitiveb	C to T at residue 403 of the coding sequence, changing residue 135 from glutamine to an amber stop codon	Brian Green and Joachim Li, unpublished results
ura3-52	no	no		Ty1 insertion (transcribing left to right) at pos. 121	Rose and Winston 1984
ura3-1	no	yes		G to A transition at residue 701 of the coding sequence, changing residue 234 from glycine to glutamate	Yan Li, Glenn Manthey, and Adam Bailis, unpublished results
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The alleles listed below are described in Brachmann et al. 1998.

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Allele	Deleted ORF?	Reverts?	Notes	Molecular Descriptiona	Reference
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^aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1.

^bAll trp- strains are cold sensitive (Singh and Manney 1974).