|
|
| Line 19: |
Line 19: |
| | | | |
| | | | |
| − | Allele Deleted ORF? Reverts? Notes Molecular descriptiona Reference
| + | |
| − | ade1-14 no yes red colonies TGG-to-TGA nonsense change at codon 244;
| + | |
| − | GGA-to-GAA missense change at codon 185. Nakayashiki et al. 2001
| |
| − | ade2-1 no yes ochre mutation TTA-to-TTG silent change at codon 9,
| |
| − | GAA-to-TAA ochre nonsense change at codon 64,
| |
| − | AGA-to-GGA missense change at codon 101,
| |
| − | GTT-to-GTC silent change at codon 124,
| |
| − | ACG-to-ACA silent change at codon 539. Rodney Rothstein, Personal communication to SGD.
| |
| − | ade2-101 no yes ochre mutation, red colonies G to T transversion at nucleotide 190, changing amino acid 64 from a Glu to a Stop Gai and Voytas, 2005
| |
| − | ade2-BglII no no red colonies frameshift (BglII site filled in at position 592) Engebrecht and Roeder 1990
| |
| − | can1-100 no yes ochre mutation AAA-to-TAA ochre nonsense change at codon 47 Rodney Rothstein, Personal communication to SGD.
| |
| − | his3delta200 yes no Cold sensitive; high frequency of petite formation, especially during transformation.
| |
| − | Note that this deletion damages the PET56 promoter. See Zhang et al., (2003) for a discussion of this issue. 1 kb deletion, (-205 to 835) Struhl 1985; Fasullo and Davis 1988; Siram et al. YGM RNA processing mtg 1993
| |
| − | his3delta1 partial no - 187 bp HindIII-HindIII internal (305 to 492) Scherer and Davis 1979
| |
| − | his3-11,15 no no double mutant G deletion at nucleotide 208,
| |
| − | G deletion at nucleotide 319. Rodney Rothstein, Personal communication to SGD.
| |
| − | leu2delta1 partial no - 0.6 kb deletion, EcoRI-ClaI internal (163 to 649) Sikorski and Hieter 1989
| |
| − | leu2-3,112 no no double mutant GTC-to-GTT silent change at codon 56,
| |
| − | GTT-to-GCT missene change at codon 69,
| |
| − | G insertion at nucleotide 249,
| |
| − | G insertion at nucleotide 792,
| |
| − | GTT-to-GTC silent change at codon 299,
| |
| − | GAC-to-AAC missense change at codon 300. Hinnen et al. 1978;
| |
| − | Gaber and Culbertson 1982;
| |
| − | Meira LB et al., 1995;
| |
| − | Rodney Rothstein, Personal communication to SGD.
| |
| − | lys2-801 no yes amber mutation - -
| |
| − | lys2delta202 partial no - 1.0 kb deletion, XhoI-HpaI internal (1813 to 2864) Winston et al. 1995
| |
| − | trp1delta1 yes no cold sensitiveb, weak galactose inducer (deletes GAL3 UAS), removes ARS1, also called trp1-901 1.45 kb deletion, EcoRI-EcoRI (-102 to 1352) Sikorski and Hieter 1989
| |
| − | trp1delta63 partial no cold sensitiveb 0.6 kb deletion, EcoRI-HindIII (-102 to 513) Sikorski and Hieter 1989
| |
| − | trp1-1 no yes amber mutation GAG-to-TAG amber nonsense change at codon 83 McDonald, et al. 1997
| |
| − | trp1-289 no yes cold sensitiveb C to T at residue 403 of the coding sequence, changing residue 135 from glutamine to an amber stop codon. Brian Green and Joachim Li, unpublished results
| |
| − | ura3-52 no no - Ty1 insertion (transcribing left to right) at pos. 121 Rose and Winston 1984
| |
| − | ura3-1 no yes - G to A transition at residue 701 of the coding sequence, changing residue 234 from glycine to glutamate Yan Li, Glenn Manthey, and Adam Bailis, unpublished results
| |
| − | The following new alleles listed below are described in Brachmann et al. 1998
| |
| − | ade2delta::hisG no no - - (Aparicio et al. 1991)
| |
| − | leu2delta0 yes no designer deletion - Brachmann et al. 1998
| |
| − | lys2delta0 yes no designer deletion - Brachmann et al. 1998
| |
| − | met15delta0 yes no designer deletion - Brachmann et al. 1998
| |
| − | ura3delta0 yes no designer deletion - Brachmann et al. 1998
| |
| | aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1. | | aThe sequence coordinates are relative to the first ATG of the selectable marker ORF, in which the A residue is defined as +1. |
| | | | |
| | bAll trp- strains are cold sensitive (Singh and Manney 1974). | | bAll trp- strains are cold sensitive (Singh and Manney 1974). |
