Difference between revisions of "YDR028C"

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==Community Commentary==
 
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==References==
 
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Revision as of 08:17, 30 January 2007

Share your knowledge...Edit this entry! <protect>

Systematic name YDR028C
Gene name REG1
Aliases HEX2, PZF240, SPP43, SRN1
Feature type ORF, Verified
Coordinates Chr IV:500876..497832


Description of YDR028C: Regulatory subunit of type 1 protein phosphatase Glc7p, involved in negative regulation of glucose-repressible genes[1][2][3]




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Community Commentary

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Alleles, Strains, and Phenotypes

Complete Deletion

Allele: reg1::URA3

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4569
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)

reg1 mutants are consitutively filamentous (filamentous even in the presence of glucose, unlike wild-type, which filaments on media lacking glucose). [4] [5]

Allele: reg1::URA3

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4569
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)

reg1 mutants display a constitutive invasive phenotype (they invade better than wild-type on medium containing high glucose (2%), but about the same as wild-type in limiting glucose (0.1%)). [4] [5]

Allele: reg1::URA3

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4569
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)

reg1 mutants are consitutively filamentous (filamentous even in the presence of glucose, unlike wild-type, which filaments on media lacking glucose). [4] [5]

Allele: reg1::URA3

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4569
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)

reg1 mutants display a constitutive invasive phenotype (they invade better than wild-type on medium containing high glucose (2%), but about the same as wild-type in limiting glucose (0.1%)). [4] [5]

Allele: reg1::URA3

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4462
Together with: SNF1
Phenotype(s): Loss of function (Null)

reg1 mutants invade better than wild-type. [6] [5]

Multiple Knockout Strains

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4469
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of FLO11/MUC1 suppresses reg1's constitutive invasion phenotype, but not its constitutive filamentous phenotype. [4] [5]

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4571
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). [4] [5]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4633
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. [4] [5]

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4571
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of GAL83 suppresses reg1's constitutive adherance to plastic (adherance in high glucose (2%)). [4] [5]

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4469
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of FLO11/MUC1 suppresses reg1's constitutive invasion phenotype, but not its constitutive filamentous phenotype. [4] [5]

Strain Background: Sigma1278b (Sigma 2000 series)
Genotype: MCY4633
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null)

Deletion of both SIP2 and GAL83 in a reg1 mutant suppresses its constitutive filamentous phenotype. [4] [5]

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4618
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null), FLO11/MUC1 promoter substituted with S.p.Adh+ promoter

Deletion of REG1 in cells where FLO11/MUC1 expression is clamped (by substitution of the FLO11 promoter with the S.pombe Adh+promoter) causes an increase in invasion. [4] [5]

Strain Background: Sigma1278b
Genotype: MCY4461
Together with: SNF1
Phenotype(s): Loss of function (Null)

Deletion of SNF1 suppresses the hyperinvasive phenotype of reg1. [6] [5]

Strain Background: Sigma1278b (Sigma2000 series)
Genotype: MCY4618
Together with: SIP1, GAL83, SIP2, MUC1
Phenotype(s): Loss of function (Null), FLO11/MUC1 promoter substituted with S.p.Adh+ promoter

Deletion of REG1 in cells where FLO11/MUC1 expression is clamped (by substitution of the FLO11 promoter with the S.pombe Adh+promoter) causes an increase in invasion. [4] [5]

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References

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  1. Jump up ↑ Frederick DL and Tatchell K (1996) The REG2 gene of Saccharomyces cerevisiae encodes a type 1 protein phosphatase-binding protein that functions with Reg1p and the Snf1 protein kinase to regulate growth. Mol Cell Biol 16(6):2922-31 SGD PMID 8649403
  2. Jump up ↑ Tu J and Carlson M (1995) REG1 binds to protein phosphatase type 1 and regulates glucose repression in Saccharomyces cerevisiae. EMBO J 14(23):5939-46 SGD PMID 8846786
  3. Jump up ↑ Niederacher D and Entian KD (1987) Isolation and characterization of the regulatory HEX2 gene necessary for glucose repression in yeast. Mol Gen Genet 206(3):505-9 SGD PMID 3035346
  4. ↑ Jump up to: 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 Vyas VK, et al. (2003) Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol 23(4):1341-8 SGD PMID 12556493
  5. ↑ Jump up to: 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 5.13 submitted by Valmik K. Vyas on 2003-03-27
  6. ↑ Jump up to: 6.0 6.1 Kuchin S, et al. (2002) Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol 22(12):3994-4000 SGD PMID 12024013

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