Difference between revisions of "YMR011W"

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=== Alleles, Strains, and Phenotypes ===
 
[[Category:Topic:Alleles, Strains, and Phenotypes]]
 
==== Multiple Knockout Strains ====
 
[[Category:Topic:Alleles, Strains, and Phenotypes:Multiple Knockout Strains]]
 
 
'''Strain Background''': CEN.PK2-1C<br>
 
'''Genotype''': EBY4000 (hxt)<br>
 
'''Together with''': HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, GAL2, HXT14, HXT17, HXT11<br>
 
'''Phenotype(s)''': Viable [[Category:Phenotype:Viable]]
 
, Conditionally inviable when on glucose medium
 
 
The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. <ref name='S000056317'>Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 {{SGDpaper|S000056317}} PMID 10618490</ref> <ref name = 'CAset539-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=539 Eckhard Boles] on 2003-03-17</ref>
 
 
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=== DNA and RNA Details ===
 
[[Category:Topic:DNA and RNA Details]]
 
==== Other DNA and RNA Details ====
 
[[Category:Topic:DNA and RNA Details:Other DNA and RNA Details]]
 
'''Other Topic''': expression [[Category:Topic:expression]]
 
 
Specifically lower expression in nitrogen limited chemostat cultures versus nitrogen excess. <ref name='S000073646'>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 {{SGDpaper|S000073646}} PMID 12414795</ref> <ref name = 'CAset9153-2003-07-25'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=9153 Viktor Boer] on 2003-07-25</ref>
 
 
{{ShortCenteredHR}}
 
'''Other Topic''': expression [[Category:Topic:expression]]
 
 
Specifically higher expression in carbon limited chemostat cultures versus carbon excess. <ref name='S000073646'>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 {{SGDpaper|S000073646}} PMID 12414795</ref> <ref name = 'CAset9153-2003-07-25'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=9153 Viktor Boer] on 2003-07-25</ref>
 
 
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==Community Commentary==
 
==Community Commentary==

Revision as of 10:51, 24 January 2007

Share your knowledge...Edit this entry!

Systematic name YMR011W
Gene name HXT2
Aliases
Feature type ORF, Verified
Coordinates Chr XIII:288078..289703
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Description of YMR011W: High-affinity glucose transporter of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose[1][2]



Community Commentary

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: CEN.PK2-1C
Genotype: EBY4000 (hxt)
Together with: HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT1, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, GAL2, HXT14, HXT17, HXT11
Phenotype(s): Viable , Conditionally inviable when on glucose medium

The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. [3] [4]


DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically lower expression in nitrogen limited chemostat cultures versus nitrogen excess. [5] [6]


Other Topic: expression

Specifically higher expression in carbon limited chemostat cultures versus carbon excess. [5] [6]


References

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  1. Ozcan S and Johnston M (1995) Three different regulatory mechanisms enable yeast hexose transporter (HXT) genes to be induced by different levels of glucose. Mol Cell Biol 15(3):1564-72 SGD PMID 7862149
  2. Ozcan S and Johnston M (1999) Function and regulation of yeast hexose transporters. Microbiol Mol Biol Rev 63(3):554-69 SGD PMID 10477308
  3. Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 SGD PMID 10618490
  4. submitted by Eckhard Boles on 2003-03-17
  5. 5.0 5.1 Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  6. 6.0 6.1 submitted by Viktor Boer on 2003-07-25

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