Positions in yeast labs

Postdoctoral Research Associate at Department of Plant Sciences, University of Cambridge

Postdoctoral Research Associate in the group of Dr Janneke Balk, Department of Plant Sciences, University of Cambridge

Salary: £27,183-£35,469 pa Limit of tenure: grant funding is available for 3 years in the first instance. Start date: 1 January 2010

Applicants must have a PhD, and a background in fungal genetics will be highly preferred. Previous experience in molecular biology and biochemistry methods is essential. This project is funded by the Wellcome Trust and includes part-time technical assistance.

The assembly of iron-sulphur cofactors in respiratory complex I

Complex I deficiencies are the most common cause of respiratory chain dysfunction, but how this largest of the respiratory complexes is assembled, including its unique chain of eight iron-sulphur (Fe-S) clusters, is poorly understood. This project aims to investigate the assembly of complex I using the yeast Yarrowia lipolytica as a genetic model organism, and will focus on: 1) Further analysis of the molecular role of Ind1, a recently identified Fe-S scaffold protein important for complex I assembly in Yarrowia and human cell lines (Bych et al 2008; Sheftel et al, unpublished). 2) Development of Yarrowia yeast as a genetic model organism to perform forward genetics screens, complemented by a biochemical approach, to identify additional complex I assembly factors, in particular those that assist Ind1 with Fe-S cluster insertion. 3) Translation of the findings to human disease: identification of human orthologues and whether they are implicated in complex I disorders.

This project involves collaborations with Dr Judy Hirst at the MRC Cambridge (in vitro cluster assembly in complex I subunits), Professor Ulrich Brandt, Frankfurt (complex I function and genetics in Yarrowia yeast) and Professor Jan Smeitink, Nijmegen (translation of the findings to human respiratory disease).

Dr Janneke Balk started an independent research group in 2005, focussing on Fe-S protein biogenesis in (higher) eukaryotes. Her dynamic and international group is rapidly expanding and moving to new laboratory space this autumn.

The University of Cambridge is a world-class research institute, which is particulary strong in biomedical sciences.

Reference: Bych K, Kerscher S, Netz DJA, Pierik AJ, Zwicker K, Huynen MA, Lill R, Brandt U and Balk, J (2008). The iron-sulfur protein Ind1 is required for effective complex I assembly. EMBO J. 27, 1736-46.

For further enqueries: Janneke Balk, jb511@cam.ac.uk To apply, see http://www.plantsci.cam.ac.uk/jobs/content.html

Postdoctoral Position at University of Texas Health Science Center at San Antonio

A postdoctoral position is available in the laboratory of Dr. David Kadosh in the Department of Microbiology and Immunology at the University of Texas Health Science Center at San Antonio. Research will focus on mechanisms that determine morphology and virulence of Candida albicans, the major fungal pathogen, in response to host environmental cues (for additional details see Carlisle, et al., PNAS 106:599-604 (2009)[1], Banerjee et al., MBC 19:1354-1365 (2008)[2] and http://www.uthscsa.edu/micro/faculty/dk/dk.asp). Many opportunities are available for collaboration with both basic and clinical mycologists at the San Antonio Center for Medical Mycology (see http://www.sacmm.org/). This group represents one of the largest mycology centers in the U.S. and includes 15 laboratories working on a variety of topics in fungal pathogenesis.

Individuals with previous experience in molecular biology (including transcriptional regulation and genomics), cell biology, genetics, protein chemistry or fungal pathogenesis, as well as S. cerevisiae researchers interested in transitioning to fungal pathogenesis, are especially encouraged to apply. Please send a cover letter, CV, and contact information for three references to Melissa Olveda (olvedam@uthscsa.edu).

All postdoctoral appointments are designated as security sensitive positions. The University of Texas Health Science Center at San Antonio is an Equal Employment Opportunity/Affirmative Action Employer.

Postdoctoral or Staff Scientist Position at Purdue University

Postdoctoral/Staff scientist position available in yeast functional genomics of chromosome segregation at Purdue University. Project goals are to define, on a global scale, the effect of phosphorylation by the Aurora kinase on protein interactions controlling chromosome segregation process in yeast. Project is funded for up to five years by NIH and will involve high-throughput screening and lab automation, genome-wide studies, microscopy, yeast molecular biology and bioinformatic analysis. Applicants should have a recent PhD in any field of biology that includes a strong conceptual and experimental background in Genetics, and/or Molecular and Cell Biology.

Applicants should send a CV by email to Dr. Tony Hazbun listed at the following web site: http://www.mcmp.purdue.edu/faculty/?uid=hazbun

Postdoctoral Position at Rutgers University

A postdoctoral position is available at the Biotech Center at Rutgers University in the laboratory of Dr. Nilgun E. Tumer to investigate the mechanisms of uptake, transport and cytotoxicity of ricin and Shiga-like toxins. The Tumer lab works on elucidating the basic mechanisms of action of ricin and Shiga-like toxins using Saccharomyces cerevisae and C. elegans as model systems. Both toxins depurinate the sarcin/ricin loop of the large rRNA and inhibit translation. Our goal is to identify the targets of these toxins to understand the mechanism by which they kill cells and to develop therapeutic intervention strategies. The successful candidate will use genetic, cell biology and chemical genomic approaches to investigate the mechanism of uptake and transport of the toxins in yeast and C. elegans and use chemical genomics approaches to identify the cellular pathways affected by these toxins. The ideal candidate should have a Ph.D. in a related field, experience with yeast and/or C.elegans genetics and cell biology and familiarity with chemical biology approaches. The Biotech Center is located in New Brunswick, New Jersey near New York City and Philadelphia and is equipped with state-of-the-art facilities and instrumentation. Rutgers University is an equal opportunity employer that strongly encourages underrepresented groups to apply for open positions.

Interested applicants should send their CV and names and addresses of three references to Dr. Nilgun E. Tumer (tumer@aesop.rutgers.edu). Information on our research can be found at: http://biotech.rutgers.edu/faculty/tumer.html, http://aesop.rutgers.edu/~plantbiopath/faculty/tumer/TUMER.HTML, http://lifesci.rutgers.edu/~molbiosci/faculty/tumer.html Contact: Nilgun Tumer, Foran Hall, Biotech Center, Rutgers University, 59 Dudley Road, New Brunswick, NJ 08901-8520. Tel: 732-932-8165 X215.

Postdoctoral Position, Chemical Genomics

Research Department: Molecular Ligand Target Research Team, Chemical Genomics Research Group, RIKEN Advanced Science Institute Dr. Charles Boone, Visiting Scientist Dr. Minoru Yoshida, Team Leader

Location: RIKEN Advanced Science Institute, RIKEN Wako Institute, Hirosawa 2-1, Wako, Saitama 351-0198, Japan

Area of Research: Chemical genetics and chemical genomics

Job Description: A postdoctoral researcher position is open in the field of chemical genomics. We have constructed a unique chemical library based on natural products in the Department of Chemical Biology, RIKAN Advanced Science Institute. The successful candidate will carry out original research on global analysis of drug-target interaction using the chemical library and various yeast mutants or transformants.

Selection Process: Candidates will be selected based on research results, publications, letters of recommendation (preferably in English), etc. The CV (preferably detailed and complete) and publications must be in English.

Application Deadline: Applications are now being accepted. Position will be closed after a suitable candidate is found.

Start of Employment: Immediately

Contract Conditions: Full-time employment with the contract shall be for one year, renewable annually upon evaluation until the end of the project (March 2012). Salary will be commensurate with qualifications and experience. Commuting and housing allowances will be provided. Moving expenses to RIKEN will be reimbursed. Social insurance will also be applicable. Days off include public holidays, New Year's holidays (Dec. 29-Jan 3), summer holidays and RIKEN Foundation Day. These and other employment provisions are in accordance with RIKEN regulations.

Information at Web: Information on our team can be found on our website (http://www.riken.go.jp/engn/r-world/research/lab/asi/chemical/index.html)

Applications: Electronic copies of your curriculum vitae/résumé, a list of publications, two or three representative publications, and two reference letters should be sent to the address below or via e-mail to "charlie.boone" (add @utoronto.ca" to complete the address): Charles Boone Professor, University of Toronto, Donnelly CCBR 160 College St, Rm 1306 Toronto, ON M5S 3E1

Notes: The documents you submit will be handled with the utmost care in accordance with RIKEN's rules for the protection of personal data and will be used only for employment screening purposes. This information will not be divulged, assigned, or loaned to a third party without legitimate reason.

Postdoctoral Position: Engineering sea lamprey antibodies

A vacant postdoctoral position at the Center of Marine Biotechnology in Baltimore to characterize antigen binding properties of VLR, the unique variable lymphocyte receptors of sea lamprey. The successful candidate will join a study with the goal to explore biotechnology applications for these novel antibodies (Pancer & Mariuzza, Nat. Biotechnol., 2008; Rogozin et al., Nat. Immunol., 2007; Alder et al., Science 2005; Pancer et al., Nature 2004). Our research platform includes yeast displayed recombinant VLR libraries that are screened with various antigens. Biochemical and structural properties of monoclonal VLRs are then characterized.

Candidates must have a PhD with solid background in protein biochemistry and molecular biology, experience in recombinant protein expression and characterization, and relevant peer-reviewed publications.

To apply for position #300957 please submit a letter describing your research interests and experience, an updated curriculum vitae and names of three references via http://www.cytiva.com/umbi/ext/detail.asp?jobid=umbi300957

Contact: Zeev Pancer, PhD Center of Marine Biotechnology, UMBI Columbus Center, Suite 236 701 East Pratt St. Baltimore, MD 21202, USA Office: 410-234-8834; Fax: 410-234-8896 Email: pancer@comb.umbi.umd.edu

Epigenetic Postdoctoral Fellow Posting

Postdoctoral positions are available at Massachusetts General Hospital Cancer Center. My laboratory is focused on understanding the impact that both methylation and acetylation dynamics has in both human cell culture and C. elegans. In particular, the laboratory is investigating the impact that the histone 3 lysine 9/36 tri-demethylases [JMJD2A-D; Whetstine et al., (2007) Cell 125: 467-81] have on tumorigenesis, transcriptional regulation, and genomic integrity. The laboratory will interrogate the role of these enzymes by using genomic, proteomic, cytological and genetic approaches. Similar approaches allowed an important link to be established for histone deacetylase 1 (HDAC-1) and the regulation of extra-cellular matrix biology in both human and C. elegans, which has direct implications in cancer chemotherapy [Whetstine et al., (2005) Mol. Cell 18:483-90]. The laboratory will continue to investigate the functional overlap or unique pathways that the C. elegans class I histone deacetylases regulate by using the same type of approaches. Overall, the laboratory will integrate a number of approaches and systems to determine the important biological pathways regulated by histone demethylases and histone deacetylases.

The laboratory is looking for highly motivated, tenacious scientists that are enthusiastic, team players and love science. The laboratory is looking for researchers with documented proficiency in any of the following areas (basic molecular biology, protein biochemistry, genomics, epigenetics, C. elegans, cytology, development biology, DNA damage and repair) but interested in learning new approaches or systems to answer the exciting questions before us.

Requirements: For these positions a Ph.D. and/or M.D. is required. These positions require enthusiastic, self motivated, independent thinkers with strong interpersonal skills, and the ability to communicate with laboratory members, national and international collaborators. Please have three letters of recommendation sent to the below address and/or e-mail.

Johnathan R. Whetstine, Ph.D. Assistant Professor of Medicine Harvard Medical School and Massachusetts General Hospital Cancer Center Building 149, 13th Street , Room 7-213 Charlestown, MA 02129, USA Office Phone: 617-643-4374 jwhetstine@hms.harvard.edu

INDIANAPOLIS, Indiana University-Purdue University, Indianapolis. Postdoctoral position in DNA repair and genomic stability.

'A postdoctoral position is available for qualified graduates to pursue further training in genetics/molecular biology. We use molecular and classic genetics approaches to examine the mechanisms of DNA repair and recombination in yeast Saccharomyces cerevisiae – a model eukaryotic organism. The successful candidate will investigate genetic and environmental factors that channel repair of double-strand DNA breaks into dangerous repair pathways (break-induced replication, single-strand annealing, and breakage-fusion-bridge cycles) leading to genomic instabilities.

Applicants should hold a Ph.D. in Biology, Molecular Biology or a related life science discipline. The ideal applicant should have experience in genetics and molecular biology. The laboratory is located on the campus of Indiana University Purdue University – Indianapolis. This campus is in downtown Indianapolis, the Indiana state capital and a city of over 1 million people with outstanding resources and a low cost of living. The campus is the major academic research location in the state. Postdocs at IUPUI receive competitive compensation and benefits packages. IUPUI also offers extensive career development opportunities for our postdoctoral fellows, including a Preparing Future Faculty program. Interested persons should send curriculum vitae and the names and contact information of three references by e-mail to amalkova@iupui.edu (Dr.Anna Malkova) with "Postdoc Opening 2008" in the message title.

Seattle, Wa Fred Hutchinson Cancer Research Center

'STAFF SCIENTIST/ POSTDOCTORAL RESEARCH FELLOW '#KSW-21860

The overarching goal of the Paulovich laboratory at the Fred Hutchinson Cancer Research Center is to characterize human phenotypic variation and translate this variation into clinically useful diagnostics. The laboratory is a highly interdisciplinary collaborative setting in which chemists, biochemists, statisticians, computer scientists, biologists, and geneticists work closely together on projects ranging from basic yeast genetics to the mammalian cell DNA damage response to mass spectrometry and biomarker discovery.

Job Description: This is an upper-level, flexible position for an experienced yeast geneticist to lead a new Project in the laboratory. This position is funded off of a new R01 grant aimed to characterize cellular networks that buffer the phenotypic effects of defects in the DNA damage response pathway, using the yeast S. cerevisiae as a model system.

This position can be adapted to suit a variety of career goals, including:

• a new postdoctoral fellow (with graduate training in yeast genetics) interested in developing technical and leadership skills for a future academic position

• a Staff Scientist- or Postdoctoral-level applicant who would like a long-term position in the group and the opportunity to develop a research project

• a Staff Scientist- or Postdoctoral-level applicant who has chosen not to develop his/her own laboratory at this time, but would like the opportunity to lead a small research group within our laboratory and to co-author NIH grants. This is a unique opportunity for a go-getter who wants to take his/her career to the next level but does not plan to have an independent laboratory in the next 4-5 years. Highly motivated recent graduates are encouraged to apply.

Major duties:

Scientific: • Build new yeast strains and libraries • Perform genetic screens for mutants sensitive to DNA damage • Characterize mutants that buffer defects in the DNA damage response • Carry out yeast genetics and physiology experiments: kill curves, mutation rates, recombination rates, crosses, tetrad analysis, transformation, and genotyping

Managerial: • Assume primary responsibility for day-to-day management of the project, its budget, and its milestones • Read relevant technical literature and play a lead role in strategic planning for the project • Assist in hiring and managing staff for the project • Write manuscripts for publication • Prepare progress reports for funding agencies • Assist in / serve as PI on future grant applications • Present work at local and national meetings • Provide mentorship for junior members of the team

Qualifications: A Ph.D. and extensive experience with molecular and cellular biology techniques, as well as with YEAST MOLECULAR GENETICS, including cell mating, tetrad analysis, and physiology experiments with S. cerevisiae, are required. The successful candidate must be a highly confident self-starter who is productive when working with minimal supervision, able to read the literature critically, capable of designing experiments independently, capable of contributing intellectually to the project, and must be an effective collaborator and leader of a team.

Job Type: Full time position

Compensation: Salary DOE (or based on NIH scale for postdoctoral level) + excellent benefits

OPENING DATE: June 25, 2008; description revised 7-9-08

To apply for this position, please send cover letter, curriculum vitae, and the contact information for three professional references to:

Käthe Watanabe Human Resources Specialist Fred Hutchinson Cancer Research Center Human Resources, J1-105 P.O. Box 19024 Seattle, WA 98109-1024 Email: kwatanab@fhcrc.org Fax: 206-667-4051 Web site: http://www.fhcrc.org

University of New Mexico

Post doctoral position available now at the University of New Mexico, Albuquerque to study differentiation of quiescent and non-quiescent cells in yeast stationary phase cultures. This position is for a beginning post doc and applicants should have a strong background in yeast genetics, genomics, or flow cytometry. For recent work see Mol. Biol. Cell 2008 19: 1271-1280; and http://biology.unm.edu/biology/maggieww/public_html/Maggieww.html.

Applicants should email their cv and names/email address of three references to Maggie Werner-Washburne, maggieww@unm.edu.

Duke University Medical Center

NIH-funded postdoctoral positions are available at Duke University Medical Center to study quantitative (complex) traits in “S. cerevisiae”; for example, see Nature 416:326-330 (2002) and PLoS Genetics 2(2):e13 (2006).

Applicants should have 0 to (at most) 2 years of post-doctoral experience and a strong background in at least one of three areas – yeast genetics, quantitative/population genetics and/or genomics/informatics – and a desire to expand into the other listed areas. Start dates are flexible.

See the lab website for more information and publications. Applicants should email their curriculum vitae and the names/email addresses of three references to John McCusker. Email: mccus001@mc.duke.edu

Carnegie Institution for Science, Stanford, California, USA

Highly qualified and motivated individuals are invited to send applications for a Postdoc Position in the Research Group of Wolf B. Frommer on the topic

Regulatory circuits controlling sugar flux in yeast grown on ethanol

HT screen of the yeast knock out collection using FRET sensors for glucose, sucrose and maltose, follow-up analysis of hits and reconstruction of networks

Our lab has developed a wide range of FRET sensors for metabolites. These sensors have so far been used mainly in mammalian cells to study glutamate release from neurons, glucose transport across the ER membrane or tryptophan/kynurenine exchange.

We have now been able to functionally express the FRET sensors also in the cytoplasm of yeast and to establish a high throughput screening platform. Our goal is to identify novel regulatory pathways involved in the control of glucose flux in yeast. As a first step, the kinase k.o. collection has been screened for altered glucose flux and several hits have been identified and verified.

Next steps will be to verify the hits using a new microfluidic platform, to test the effect on other sugar fluxes using FRET sensors for sucrose, maltose and ribose and to place the kinases into signaling networks. The screen can be expanded to include the whole genome at a later stage. Focus points are regulatory effects on glucose transporters and hexokinases. Due to the advanced stage, it is expected that the work will lead to high profile publications within less than a year.

Start date asap

Send your application with CV and the names of three references to:

Wolf B. Frommer Carnegie Institution for Science 260 Panama St, Stanford CA 94305 USA. Website[3] E-mail: wfrommer@stanford.edu

B.A./B.S. Yeast Biochemist at Gevo, Inc., Englewood, CO, USA

Gevo, Inc. is a high energy, team-oriented company that is pioneering the advanced green energy industry. We are looking for a term (up to one year) Biocatalyst Development Biochemist, based in Englewood, Colorado. This role is responsible for execution of analysis of metabolites on yeast biocatalysts under the direction of a senior scientist. This person will characterize yeast biocatalysts for their intra- and extracellular metabolite production, genotypes, and phenotypes. This position is considered a term position for up to one year.

Requirements include: Bachelor degree in Biochemistry, Chemistry, Biotechnology, Microbiology or related field and 1 – 3 years of professional work experience in a lab, working with biochemical techniques and yeast. A detailed job description can be found at [4]