Positions in yeast labs

Seattle, Wa Fred Hutchinson Cancer Research Center

'STAFF SCIENTIST/ POSTDOCTORAL RESEARCH FELLOW '#KSW-21860

The overarching goal of the Paulovich laboratory at the Fred Hutchinson Cancer Research Center is to characterize human phenotypic variation and translate this variation into clinically useful diagnostics. The laboratory is a highly interdisciplinary collaborative setting in which chemists, biochemists, statisticians, computer scientists, biologists, and geneticists work closely together on projects ranging from basic yeast genetics to the mammalian cell DNA damage response to mass spectrometry and biomarker discovery.

Job Description: This is an upper-level, flexible position for an experienced yeast geneticist to lead a new Project in the laboratory. This position is funded off of a new R01 grant aimed to characterize cellular networks that buffer the phenotypic effects of defects in the DNA damage response pathway, using the yeast S. cerevisiae as a model system.

This position can be adapted to suit a variety of career goals, including:

• a new postdoctoral fellow (with graduate training in yeast genetics) interested in developing technical and leadership skills for a future academic position

• a Staff Scientist- or Postdoctoral-level applicant who would like a long-term position in the group and the opportunity to develop a research project

• a Staff Scientist- or Postdoctoral-level applicant who has chosen not to develop his/her own laboratory at this time, but would like the opportunity to lead a small research group within our laboratory and to co-author NIH grants. This is a unique opportunity for a go-getter who wants to take his/her career to the next level but does not plan to have an independent laboratory in the next 4-5 years. Highly motivated recent graduates are encouraged to apply.

Major duties:

Scientific: • Build new yeast strains and libraries • Perform genetic screens for mutants sensitive to DNA damage • Characterize mutants that buffer defects in the DNA damage response • Carry out yeast genetics and physiology experiments: kill curves, mutation rates, recombination rates, crosses, tetrad analysis, transformation, and genotyping

Managerial: • Assume primary responsibility for day-to-day management of the project, its budget, and its milestones • Read relevant technical literature and play a lead role in strategic planning for the project • Assist in hiring and managing staff for the project • Write manuscripts for publication • Prepare progress reports for funding agencies • Assist in / serve as PI on future grant applications • Present work at local and national meetings • Provide mentorship for junior members of the team

Qualifications: A Ph.D. and extensive experience with molecular and cellular biology techniques, as well as with YEAST MOLECULAR GENETICS, including cell mating, tetrad analysis, and physiology experiments with S. cerevisiae, are required. The successful candidate must be a highly confident self-starter who is productive when working with minimal supervision, able to read the literature critically, capable of designing experiments independently, capable of contributing intellectually to the project, and must be an effective collaborator and leader of a team.

Job Type: Full time position

Compensation: Salary DOE (or based on NIH scale for postdoctoral level) + excellent benefits

OPENING DATE: June 25, 2008; description revised 7-9-08

To apply for this position, please send cover letter, curriculum vitae, and the contact information for three professional references to:

Käthe Watanabe Human Resources Specialist Fred Hutchinson Cancer Research Center Human Resources, J1-105 P.O. Box 19024 Seattle, WA 98109-1024 Email: kwatanab@fhcrc.org Fax: 206-667-4051 Web site: http://www.fhcrc.org

University of New Mexico

Post doctoral position available now at the University of New Mexico, Albuquerque to study differentiation of quiescent and non-quiescent cells in yeast stationary phase cultures. This position is for a beginning post doc and applicants should have a strong background in yeast genetics, genomics, or flow cytometry. For recent work see Mol. Biol. Cell 2008 19: 1271-1280; and http://biology.unm.edu/biology/maggieww/public_html/Maggieww.html.

Applicants should email their cv and names/email address of three references to Maggie Werner-Washburne, maggieww@unm.edu.

Duke University Medical Center

NIH-funded postdoctoral positions are available at Duke University Medical Center to study quantitative (complex) traits in “S. cerevisiae”; for example, see Nature 416:326-330 (2002) and PLoS Genetics 2(2):e13 (2006).

Applicants should have 0 to (at most) 2 years of post-doctoral experience and a strong background in at least one of three areas – yeast genetics, quantitative/population genetics and/or genomics/informatics – and a desire to expand into the other listed areas. Start dates are flexible.

See the lab website for more information and publications. Applicants should email their curriculum vitae and the names/email addresses of three references to John McCusker. Email: mccus001@mc.duke.edu

Carnegie Institution for Science, Stanford, California, USA

Highly qualified and motivated individuals are invited to send applications for a Postdoc Position in the Research Group of Wolf B. Frommer on the topic

Regulatory circuits controlling sugar flux in yeast grown on ethanol

HT screen of the yeast knock out collection using FRET sensors for glucose, sucrose and maltose, follow-up analysis of hits and reconstruction of networks

Our lab has developed a wide range of FRET sensors for metabolites. These sensors have so far been used mainly in mammalian cells to study glutamate release from neurons, glucose transport across the ER membrane or tryptophan/kynurenine exchange.

We have now been able to functionally express the FRET sensors also in the cytoplasm of yeast and to establish a high throughput screening platform. Our goal is to identify novel regulatory pathways involved in the control of glucose flux in yeast. As a first step, the kinase k.o. collection has been screened for altered glucose flux and several hits have been identified and verified.

Next steps will be to verify the hits using a new microfluidic platform, to test the effect on other sugar fluxes using FRET sensors for sucrose, maltose and ribose and to place the kinases into signaling networks. The screen can be expanded to include the whole genome at a later stage. Focus points are regulatory effects on glucose transporters and hexokinases. Due to the advanced stage, it is expected that the work will lead to high profile publications within less than a year.

Start date asap

Send your application with CV and the names of three references to:

Wolf B. Frommer Carnegie Institution for Science 260 Panama St, Stanford CA 94305 USA. Website[1] E-mail: wfrommer@stanford.edu

Stony Brook University

A Postdoctoral position available to study the regulation of signaling and morphogenesis in Candida albicans. The primary responsibility is to develop an independent research project focused on studying the mechanisms by which this organism responds to extracellular signals to switch from budding to hyphal growth. Specific research efforts will focus on studying the role of plasma membrane organization in morphogenesis and virulence factor production. Recent references related to this project: [2] [3] [4] [5]

Research will be carried out in the Center for Yeast Molecular Genetics and Cell Biology, which includes 8 labs working on different aspects of fungal biology.

Applicants should have a strong background in genetics, biochemistry, and cell biology. Prior experience in working with yeast or fungi is preferred, but not essential.

Applicants should email their CV and contact information for three references (including email address) to James Konopka jkonopka@ms.cc.sunysb.edu

James Konopka, Ph.D. Professor Department of Molecular Genetics and Microbiology Stony Brook University Stony Brook, NY 11794-5222